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New page: left|200px<br /><applet load="1mey" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mey, resolution 2.200Å" /> '''CRYSTAL STRUCTURE O...
 
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[[Image:1mey.gif|left|200px]]<br /><applet load="1mey" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1mey, resolution 2.200&Aring;" />
'''CRYSTAL STRUCTURE OF A DESIGNED ZINC FINGER PROTEIN BOUND TO DNA'''<br />


==Overview==
==CRYSTAL STRUCTURE OF A DESIGNED ZINC FINGER PROTEIN BOUND TO DNA==
Considerable recent effort has been devoted to the design and selection of, sequence-specific DNA binding proteins based on tandem arrays of Cys2His2, zinc finger domains. While the DNA binding properties of these designed, proteins have been studied extensively, the structural basis for, site-specific binding has not been examined experimentally. Here we report, the crystal structure of a complex between a protein comprised of three, consensus-sequence-based zinc finger domains and an oligonucleotide, corresponding to a favourable DNA binding site. This structure reveals, relatively simple modular interactions and structural adaptations that, compensate for differences in contact residue side-chain lengths.
<StructureSection load='1mey' size='340' side='right'caption='[[1mey]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1mey]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MEY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MEY FirstGlance]. <br>
1MEY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with ZN and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MEY OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C38:5-IODO-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>C38</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mey OCA], [https://pdbe.org/1mey PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mey RCSB], [https://www.ebi.ac.uk/pdbsum/1mey PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mey ProSAT]</span></td></tr>
A 2.2 A resolution crystal structure of a designed zinc finger protein bound to DNA., Kim CA, Berg JM, Nat Struct Biol. 1996 Nov;3(11):940-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8901872 8901872]
</table>
[[Category: ]]
== Evolutionary Conservation ==
[[Category: Protein complex]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Berg, J.M.]]
Check<jmol>
[[Category: Kim, C.A.]]
  <jmolCheckbox>
[[Category: CL]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/me/1mey_consurf.spt"</scriptWhenChecked>
[[Category: ZN]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: complex (zinc finger/dna)]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: crystal structure]]
  </jmolCheckbox>
[[Category: protein design]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mey ConSurf].
[[Category: protein-dna interaction]]
<div style="clear:both"></div>
[[Category: zinc finger]]
__TOC__
 
</StructureSection>
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 04:09:15 2007''
[[Category: Large Structures]]
[[Category: Berg JM]]
[[Category: Kim CA]]

Latest revision as of 07:43, 14 February 2024

CRYSTAL STRUCTURE OF A DESIGNED ZINC FINGER PROTEIN BOUND TO DNA

1mey, resolution 2.20Å

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