1mis: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: left|200px<br /><applet load="1mis" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mis" /> '''STRUCTURE OF RNA (5'-R(GP*CP*GP*GP*AP*CP*GP*... |
No edit summary |
||
| (13 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
== | ==STRUCTURE OF RNA (5'-R(GP*CP*GP*GP*AP*CP*GP*C)-3') ANTI-PARALLEL RNA DUPLEX WITH TANDEM G:A MISMATCHES, NMR, MINIMIZED AVERAGE STRUCTURE== | ||
The three-dimensional solution structure of the RNA self-complementary | <StructureSection load='1mis' size='340' side='right'caption='[[1mis]]' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1mis]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MIS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MIS FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mis FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mis OCA], [https://pdbe.org/1mis PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mis RCSB], [https://www.ebi.ac.uk/pdbsum/1mis PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mis ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The three-dimensional solution structure of the RNA self-complementary duplex [sequence: see text] was derived from two-dimensional NMR and the iterative relaxation matrix approach. Each GA mismatch forms two hydrogen bonds: A-NH6 to G-O6 and A-N1 to G-NH1 (imino). This is the first three-dimensional RNA structure with imino hydrogen-bonded tandem GA mismatches. This GA structure is totally different from the sheared tandem GA structure in [sequence: see text] which also has two hydrogen bonds: A-N7 to G-NH2 and A-NH6 to G-N3 [SantaLucia, J., Jr., & Turner, D. H. (1993) Biochemistry 32, 12612-12623]. In particular, the sheared and imino GA mismatches produce a narrowing and widening of the backbone, respectively. The results show that substitutions of Watson-Crick base pairs can have dramatic effects on the three-dimensional structures of adjacent non-Watson-Crick paired regions; i.e., the structure depends on sequence context. Thus compensating substitutions in site-directed mutagenesis experiments may not always restore biological activities. | |||
Solution structure of (rGCGGACGC)2 by two-dimensional NMR and the iterative relaxation matrix approach.,Wu M, Turner DH Biochemistry. 1996 Jul 30;35(30):9677-89. PMID:8703939<ref>PMID:8703939</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
[[Category: | <div class="pdbe-citations 1mis" style="background-color:#fffaf0;"></div> | ||
[[Category: Turner | == References == | ||
[[Category: Wu | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Turner DH]] | |||
[[Category: Wu M]] | |||
Latest revision as of 18:50, 29 November 2023
STRUCTURE OF RNA (5'-R(GP*CP*GP*GP*AP*CP*GP*C)-3') ANTI-PARALLEL RNA DUPLEX WITH TANDEM G:A MISMATCHES, NMR, MINIMIZED AVERAGE STRUCTURE
| ||||||||||||
