2wzl: Difference between revisions

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{{Seed}}
[[Image:2wzl.png|left|200px]]


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==The Structure of the N-RNA Binding Domain of the Mokola virus Phosphoprotein==
The line below this paragraph, containing "STRUCTURE_2wzl", creates the "Structure Box" on the page.
<StructureSection load='2wzl' size='340' side='right'caption='[[2wzl]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2wzl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mokola_lyssavirus Mokola lyssavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WZL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WZL FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
{{STRUCTURE_2wzl|  PDB=2wzl  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wzl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wzl OCA], [https://pdbe.org/2wzl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wzl RCSB], [https://www.ebi.ac.uk/pdbsum/2wzl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wzl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PHOSP_MOKV PHOSP_MOKV] Non catalytic polymerase cofactor and regulatory protein that plays a role in viral transcription and replication. Stabilizes the RNA polymerase L to the N-RNA template and binds the soluble protein N, preventing it from encapsidating non-genomic RNA. Also inhibits host IFN-alpha and IFN-beta signaling by binding and retaining phosphorylated STAT1 in the cytoplasm or by inhibiting the DNA binding of STAT1 in the nucleus (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wz/2wzl_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wzl ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Mokola virus (MOKV) is a nonsegmented, negative-sense RNA virus that belongs to the Lyssavirus genus and Rhabdoviridae family. MOKV phosphoprotein P is an essential component of the replication and transcription complex and acts as a cofactor for the viral RNA-dependent RNA polymerase. P recruits the viral polymerase to the nucleoprotein-bound viral RNA (N-RNA) via an interaction between its C-terminal domain and the N-RNA complex. Here we present a structure for this domain of MOKV P, obtained by expression of full-length P in Escherichia coli, which was subsequently truncated during crystallization. The structure has a high degree of homology with P of rabies virus, another member of Lyssavirus genus, and to a lesser degree with P of vesicular stomatitis virus (VSV), a member of the related Vesiculovirus genus. In addition, analysis of the crystal packing of this domain reveals a potential binding site for the nucleoprotein N. Using both site-directed mutagenesis and yeast two-hybrid experiments to measure P-N interaction, we have determined the relative roles of key amino acids involved in this interaction to map the region of P that binds N. This analysis also reveals a structural relationship between the N-RNA binding domain of the P proteins of the Rhabdoviridae and the Paramyxoviridae.


===THE STRUCTURE OF THE N-RNA BINDING DOMAIN OF THE MOKOLA VIRUS PHOSPHOPROTEIN===
Structure of the nucleoprotein binding domain of Mokola virus phosphoprotein.,Assenberg R, Delmas O, Ren J, Vidalain PO, Verma A, Larrous F, Graham SC, Tangy F, Grimes JM, Bourhy H J Virol. 2010 Jan;84(2):1089-96. Epub 2009 Nov 11. PMID:19906936<ref>PMID:19906936</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2wzl" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 19906936 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_19906936}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
2WZL is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mokola_virus Mokola virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WZL OCA].
[[Category: Mokola lyssavirus]]
 
[[Category: Assenberg R]]
==Reference==
[[Category: Bourhy H]]
<ref group="xtra">PMID:19906936</ref><references group="xtra"/>
[[Category: Delmas O]]
[[Category: Mokola virus]]
[[Category: Graham S]]
[[Category: Assenberg, R.]]
[[Category: Grimes J]]
[[Category: Bourhy, H.]]
[[Category: Larrous F]]
[[Category: Delmas, O.]]
[[Category: Ren J]]
[[Category: Graham, S.]]
[[Category: Tangy F]]
[[Category: Grimes, J.]]
[[Category: Verma A]]
[[Category: Larrous, F.]]
[[Category: Vidalain P]]
[[Category: Ren, J.]]
[[Category: Tangy, F.]]
[[Category: Verma, A.]]
[[Category: Vidalain, P.]]
[[Category: Chaperone]]
[[Category: Nucleoprotein]]
[[Category: Phosphoprotein]]
[[Category: Rabies]]
[[Category: Rna replication]]
[[Category: Viral protein]]
[[Category: Virion]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 13 13:08:12 2010''
Retrieved from "https://proteopedia.org/2wzl"