1v5x: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
New page: left|200px<br /><applet load="1v5x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1v5x, resolution 2.00Å" /> '''Crystal structure of...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
 
(16 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1v5x.jpg|left|200px]]<br /><applet load="1v5x" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1v5x, resolution 2.00&Aring;" />
'''Crystal structure of Phosphoribosyl anthranilate isomerase from Thermus Thermophilus'''<br />


==Overview==
==Crystal structure of Phosphoribosyl anthranilate isomerase from Thermus Thermophilus==
The crystal structure of phosphoribosyl anthranilate isomerase (PRAI) from, Thermus thermophilus HB8 (TtPRAI) was solved at 2.0 A resolution. The, overall structure of TtPRAI with a dimeric structure was quite similar to, that of PRAI from Thermotoga maritima (TmPRAI). In order to elucidate the, stabilization mechanism of TtPRAI, its physicochemical properties were, examined using DSC, CD, and analytical centrifugation at various pHs in, relation to the association-dissociation of the subunits. Based on the, experimental results for TtPRAI and the structural information on TtPRAI, and TmPRAI, we found that: (i) the denaturation of TtPRAI at acidic pH is, correlated with the dissociation of its dimeric form; (ii) the hydrophobic, interaction of TtPRAI in the monomer structure is slightly greater than, that of TmPRAI, but dimer interface of the TmPRAI is remarkably greater;, (iii) the contributions of hydrogen bonds and ion bonds to the stability, are similar to each other; and (iv) destabilization due to the presence of, cavities in TtPRAI is greater than that of TmPRAI in both the monomer and, dimer structures.
<StructureSection load='1v5x' size='340' side='right'caption='[[1v5x]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1v5x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1V5X FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1v5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v5x OCA], [https://pdbe.org/1v5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1v5x RCSB], [https://www.ebi.ac.uk/pdbsum/1v5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1v5x ProSAT], [https://www.topsan.org/Proteins/RSGI/1v5x TOPSAN]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/P83825_THETH P83825_THETH]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v5/1v5x_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1v5x ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of phosphoribosyl anthranilate isomerase (PRAI) from Thermus thermophilus HB8 (TtPRAI) was solved at 2.0 A resolution. The overall structure of TtPRAI with a dimeric structure was quite similar to that of PRAI from Thermotoga maritima (TmPRAI). In order to elucidate the stabilization mechanism of TtPRAI, its physicochemical properties were examined using DSC, CD, and analytical centrifugation at various pHs in relation to the association-dissociation of the subunits. Based on the experimental results for TtPRAI and the structural information on TtPRAI and TmPRAI, we found that: (i) the denaturation of TtPRAI at acidic pH is correlated with the dissociation of its dimeric form; (ii) the hydrophobic interaction of TtPRAI in the monomer structure is slightly greater than that of TmPRAI, but dimer interface of the TmPRAI is remarkably greater; (iii) the contributions of hydrogen bonds and ion bonds to the stability are similar to each other; and (iv) destabilization due to the presence of cavities in TtPRAI is greater than that of TmPRAI in both the monomer and dimer structures.


==About this Structure==
Stabilization due to dimer formation of phosphoribosyl anthranilate isomerase from Thermus thermophilus HB8: X-ray Analysis and DSC experiments.,Taka J, Ogasahara K, Jeyakanthan J, Kunishima N, Kuroishi C, Sugahara M, Yokoyama S, Yutani K J Biochem. 2005 May;137(5):569-78. PMID:15944409<ref>PMID:15944409</ref>
1V5X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Active as [http://en.wikipedia.org/wiki/Phosphoribosylanthranilate_isomerase Phosphoribosylanthranilate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.24 5.3.1.24] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1V5X OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Stabilization due to dimer formation of phosphoribosyl anthranilate isomerase from Thermus thermophilus HB8: X-ray Analysis and DSC experiments., Taka J, Ogasahara K, Jeyakanthan J, Kunishima N, Kuroishi C, Sugahara M, Yokoyama S, Yutani K, J Biochem (Tokyo). 2005 May;137(5):569-78. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15944409 15944409]
</div>
[[Category: Phosphoribosylanthranilate isomerase]]
<div class="pdbe-citations 1v5x" style="background-color:#fffaf0;"></div>
[[Category: Single protein]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
[[Category: Kunishima, N.]]
[[Category: Kunishima N]]
[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
[[Category: Taka J]]
[[Category: Taka, J.]]
[[Category: Yutani K]]
[[Category: Yutani, K.]]
[[Category: alpha-beta barrel]]
[[Category: isomerase]]
[[Category: phosphoribosyl anthranilate isomerase]]
[[Category: riken structural genomics/proteomics initiative]]
[[Category: rsgi]]
[[Category: structural genomics]]
[[Category: trpf]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 04:37:51 2007''

Latest revision as of 07:47, 25 October 2023

Crystal structure of Phosphoribosyl anthranilate isomerase from Thermus Thermophilus

1v5x, resolution 2.00Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1v5x&oldid=3921934"