3a58: Difference between revisions

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{{Seed}}
[[Image:3a58.jpg|left|200px]]


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==Crystal structure of Sec3p - Rho1p complex from Saccharomyces cerevisiae==
The line below this paragraph, containing "STRUCTURE_3a58", creates the "Structure Box" on the page.
<StructureSection load='3a58' size='340' side='right'caption='[[3a58]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3a58]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A58 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A58 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
{{STRUCTURE_3a58|  PDB=3a58  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a58 OCA], [https://pdbe.org/3a58 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a58 RCSB], [https://www.ebi.ac.uk/pdbsum/3a58 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a58 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SEC3_YEAST SEC3_YEAST] Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a5/3a58_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3a58 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The exocyst complex is a hetero-octameric protein complex that functions during cell polarization by tethering the secretory vesicle to the target membrane. The yeast exocyst subunit Sec3 binds to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P(2)) and the small GTPases Rho1 and Cdc42 via its N-terminal domain (Sec3-N), and these interactions target Sec3 to the plasma membrane. Here we report the crystal structure of the Sec3-N in complex with Rho1 at 2.6-A resolution. Sec3-N adopts a pleckstrin homology (PH) fold, despite having no detectable sequence homology with other PH domains of known structure. Clusters of conserved basic residues constitute a positively charged cleft, which was identified as a binding site for PtdIns(4,5)P(2). Residues Phe77, Ile115 and Leu131 of Sec3 bind to an extended hydrophobic surface formed around switch regions I and II of Rho1. To our knowledge, these are the first structural insights into how an exocyst subunit might interact with both protein and phospholipid factors on the target membrane.


===Crystal structure of Sec3p - Rho1p complex from Saccharomyces cerevisiae===
Structural basis for the Rho- and phosphoinositide-dependent localization of the exocyst subunit Sec3.,Yamashita M, Kurokawa K, Sato Y, Yamagata A, Mimura H, Yoshikawa A, Sato K, Nakano A, Fukai S Nat Struct Mol Biol. 2010 Feb;17(2):180-6. Epub 2010 Jan 10. PMID:20062059<ref>PMID:20062059</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3a58" style="background-color:#fffaf0;"></div>


==About this Structure==
==See Also==
3A58 is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A58 OCA].
*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Fukai, S.]]
[[Category: Fukai S]]
[[Category: Mimura, H.]]
[[Category: Mimura H]]
[[Category: Sato, Y.]]
[[Category: Sato Y]]
[[Category: Yamagata, A.]]
[[Category: Yamagata A]]
[[Category: Yamashita, M.]]
[[Category: Yamashita M]]
[[Category: Yoshikawa, A.]]
[[Category: Yoshikawa A]]
[[Category: Acetylation]]
[[Category: Cell membrane]]
[[Category: Coiled coil]]
[[Category: Endosome]]
[[Category: Exocytosis]]
[[Category: Gtp-binding]]
[[Category: Gtpase]]
[[Category: Lipoprotein]]
[[Category: Membrane]]
[[Category: Membrane traffic]]
[[Category: Methylation]]
[[Category: Nucleotide-binding]]
[[Category: Peroxisome]]
[[Category: Ph domain]]
[[Category: Phosphoprotein]]
[[Category: Prenylation]]
[[Category: Protein complex]]
[[Category: Protein transport]]
[[Category: Protein transport/exocytosis complex]]
[[Category: Transport]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 13 14:20:14 2010''
Retrieved from "https://proteopedia.org/3a58"