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New page: left|200px<br /><applet load="1zb6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zb6, resolution 1.95Å" /> '''Co-Crystal Structure...
 
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[[Image:1zb6.gif|left|200px]]<br /><applet load="1zb6" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1zb6, resolution 1.95&Aring;" />
'''Co-Crystal Structure of ORF2 an Aromatic Prenyl Transferase from Streptomyces sp. strain cl190 complexed with GSPP and 1,6-dihydroxynaphtalene'''<br />


==Overview==
==Co-Crystal Structure of ORF2 an Aromatic Prenyl Transferase from Streptomyces sp. strain cl190 complexed with GSPP and 1,6-dihydroxynaphtalene==
The anti-oxidant naphterpin is a natural product containing a, polyketide-based aromatic core with an attached 10-carbon geranyl group, derived from isoprenoid (terpene) metabolism. Hybrid natural products such, as naphterpin that contain 5-carbon (dimethylallyl), 10-carbon (geranyl), or 15-carbon (farnesyl) isoprenoid chains possess biological activities, distinct from their non-prenylated aromatic precursors. These hybrid, natural products represent new anti-microbial, anti-oxidant, anti-inflammatory, anti-viral and anti-cancer compounds. A small number of, aromatic prenyltransferases (PTases) responsible for prenyl group, attachment have only recently been isolated and characterized. Here we, report the gene identification, biochemical characterization and, high-resolution X-ray crystal structures of an architecturally novel, aromatic PTase, Orf2 from Streptomyces sp. strain CL190, with substrates, and substrate analogues bound. In vivo, Orf2 attaches a geranyl group to a, 1,3,6,8-tetrahydroxynaphthalene-derived polyketide during naphterpin, biosynthesis. In vitro, Orf2 catalyses carbon-carbon-based and, carbon-oxygen-based prenylation of a diverse collection of, hydroxyl-containing aromatic acceptors of synthetic, microbial and plant, origin. These crystal structures, coupled with in vitro assays, provide a, basis for understanding and potentially manipulating the regio-specific, prenylation of aromatic small molecules using this structurally unique, family of aromatic PTases.
<StructureSection load='1zb6' size='340' side='right'caption='[[1zb6]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1zb6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp. Streptomyces sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZB6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZB6 FirstGlance]. <br>
1ZB6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Streptomyces_sp. Streptomyces sp.] with MG, NO3, GST and DIN as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZB6 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DIN:1,6-DIHYDROXY+NAPHTHALENE'>DIN</scene>, <scene name='pdbligand=GST:GERANYL+S-THIOLODIPHOSPHATE'>GST</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zb6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zb6 OCA], [https://pdbe.org/1zb6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zb6 RCSB], [https://www.ebi.ac.uk/pdbsum/1zb6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zb6 ProSAT]</span></td></tr>
Structural basis for the promiscuous biosynthetic prenylation of aromatic natural products., Kuzuyama T, Noel JP, Richard SB, Nature. 2005 Jun 16;435(7044):983-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15959519 15959519]
</table>
[[Category: Protein complex]]
== Function ==
[[Category: Streptomyces sp.]]
[https://www.uniprot.org/uniprot/Q4R2T2_STRC1 Q4R2T2_STRC1]  
[[Category: Kuzuyama, T.]]
== Evolutionary Conservation ==
[[Category: Noel, J.P.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Richard, S.B.]]
Check<jmol>
[[Category: DIN]]
  <jmolCheckbox>
[[Category: GST]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zb/1zb6_consurf.spt"</scriptWhenChecked>
[[Category: MG]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: NO3]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: novel aromatic prenyltransferase barrel fold]]
  </jmolCheckbox>
[[Category: pt-barrel]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zb6 ConSurf].
 
<div style="clear:both"></div>
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 04:48:21 2007''
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces sp]]
[[Category: Kuzuyama T]]
[[Category: Noel JP]]
[[Category: Richard SB]]
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