2x1p: Difference between revisions
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==Gelsolin Nanobody== | |||
<StructureSection load='2x1p' size='340' side='right'caption='[[2x1p]], [[Resolution|resolution]] 1.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2x1p]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X1P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X1P FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x1p OCA], [https://pdbe.org/2x1p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x1p RCSB], [https://www.ebi.ac.uk/pdbsum/2x1p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x1p ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
RNA interference has tremendously advanced our understanding of gene function but recent reports have exposed undesirable side-effects. Recombinant Camelid single-domain antibodies (VHHs) provide an attractive means for studying protein function without affecting gene expression. We raised VHHs against gelsolin (GsnVHHs), a multifunctional actin-binding protein that controls cellular actin organization and migration. GsnVHH-induced delocalization of gelsolin to mitochondria or the nucleus in mammalian cells reveals distinct subpopulations including free gelsolin and actin-bound gelsolin complexes. GsnVHH 13 specifically recognizes Ca(2+)-activated gelsolin (K (d) approximately 10 nM) while GsnVHH 11 binds gelsolin irrespective of Ca(2+) (K (d) approximately 5 nM) but completely blocks its interaction with G-actin. Both GsnVHHs trace gelsolin in membrane ruffles of EGF-stimulated MCF-7 cells and delay cell migration without affecting F-actin severing/capping or actin nucleation activities by gelsolin. We conclude that VHHs represent a potent way of blocking structural proteins and that actin nucleation by gelsolin is more complex than previously anticipated. | |||
A llama-derived gelsolin single-domain antibody blocks gelsolin-G-actin interaction.,Van den Abbeele A, De Clercq S, De Ganck A, De Corte V, Van Loo B, Soror SH, Srinivasan V, Steyaert J, Vandekerckhove J, Gettemans J Cell Mol Life Sci. 2010 May;67(9):1519-35. Epub 2010 Feb 7. PMID:20140750<ref>PMID:20140750</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2x1p" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Gelsolin 3D structures|Gelsolin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Lama glama]] | |||
[[Category: Large Structures]] | |||
[[Category: De Corte V]] | |||
[[Category: De Ganck A]] | |||
[[Category: Declercq S]] | |||
[[Category: Gettemans J]] | |||
[[Category: Srinivasan V]] | |||
[[Category: Steyaert J]] | |||
[[Category: Van De Kerckhove J]] | |||
[[Category: Van Den Abbeele A]] | |||
[[Category: Van Loo B]] | |||
