3lpv: Difference between revisions
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New page: '''Unreleased structure''' The entry 3lpv is ON HOLD Authors: Todd, R.C., Lippard, S.J. Description: X-ray crystal structure of duplex DNA containing a cisplatin 1,2-d(GpG) intrastrand... |
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The | ==X-ray crystal structure of duplex DNA containing a cisplatin 1,2-d(GpG) intrastrand cross-link== | ||
<StructureSection load='3lpv' size='340' side='right'caption='[[3lpv]], [[Resolution|resolution]] 1.77Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3lpv]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LPV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LPV FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.77Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CPT:CISPLATIN'>CPT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3lpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lpv OCA], [https://pdbe.org/3lpv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3lpv RCSB], [https://www.ebi.ac.uk/pdbsum/3lpv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3lpv ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We report the 1.77-A resolution X-ray crystal structure of a dodecamer DNA duplex with the sequence 5'-CCTCTGGTCTCC-3' that has been modified to contain a single engineered 1,2-cis-{Pt(NH(3))(2)}(2+)-d(GpG) cross-link, the major DNA adduct of cisplatin. These data represent a significant improvement in resolution over the previously published 2.6-A structure. The ammine ligands in this structure are clearly resolved, leading to improved visualization of the cross-link geometry with respect to both the platinum center and to the nucleobases, which adopt a higher energy conformation. Also better resolved are the deoxyribose sugar puckers, which allow us to re-examine the global structure of platinum-modified DNA. Another new feature of this model is the location of four octahedral [Mg(H(2)O)(6)](2+) ions associated with bases in the DNA major groove and the identification of 124 ordered water molecules that participate in hydrogen-bonding interactions with either the nucleic acid or the diammineplatinum(II) moiety. | |||
Structure of duplex DNA containing the cisplatin 1,2-{Pt(NH3)2}2+-d(GpG) cross-link at 1.77 A resolution.,Todd RC, Lippard SJ J Inorg Biochem. 2010 Sep;104(9):902-8. Epub 2010 Apr 18. PMID:20541266<ref>PMID:20541266</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3lpv" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Lippard SJ]] | |||
[[Category: Todd RC]] | |||
Latest revision as of 08:42, 6 September 2023
X-ray crystal structure of duplex DNA containing a cisplatin 1,2-d(GpG) intrastrand cross-link
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