2kuy: Difference between revisions
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New page: '''Unreleased structure''' The entry 2kuy is ON HOLD Authors: Venugopal, H., Edwards, P., Schwalbe, M., Claridge, J., Stepper, J., Patchett, M., Loo, T., Libich, D., Norris, G., Pascal,... |
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The | ==Structure of Glycocin F== | ||
<StructureSection load='2kuy' size='340' side='right'caption='[[2kuy]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2kuy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactiplantibacillus_plantarum Lactiplantibacillus plantarum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KUY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KUY FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 12 models</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kuy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kuy OCA], [https://pdbe.org/2kuy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kuy RCSB], [https://www.ebi.ac.uk/pdbsum/2kuy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kuy ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/GCCF_LACPN GCCF_LACPN] Has antibacterial activity against L.plantarum ATCC 8014. In purified form, the activity is bacteriostatic (IC(50)=2 nM) rather than bactericidal.<ref>PMID:21251913</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Bacteriocins are bacterial peptides with specific activity against competing species. They hold great potential as natural preservatives and for their probiotic effects. We show here nuclear magnetic resonance-based evidence that glycocin F, a 43-amino acid bacteriocin from Lactobacillus plantarum, contains two beta-linked N-acetylglucosamine moieties, attached via side chain linkages to a serine via oxygen, and to a cysteine via sulfur. The latter linkage is novel and has helped to establish a new type of post-translational modification, the S-linked sugar. The peptide conformation consists primarily of two alpha-helices held together by a pair of nested disulfide bonds. The serine-linked sugar is positioned on a short loop sequentially connecting the two helices, while the cysteine-linked sugar presents at the end of a long disordered C-terminal tail. The differing chemical and conformational stabilities of the two N-actetylglucosamine moieties provide clues about the possible mode of action of this bacteriostatic peptide. | |||
Structural, dynamic, and chemical characterization of a novel s-glycosylated bacteriocin.,Venugopal H, Edwards PJ, Schwalbe M, Claridge JK, Libich DS, Stepper J, Loo T, Patchett ML, Norris GE, Pascal SM Biochemistry. 2011 Apr 12;50(14):2748-55. Epub 2011 Mar 21. PMID:21395300<ref>PMID:21395300</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2kuy" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Lactiplantibacillus plantarum]] | |||
[[Category: Large Structures]] | |||
[[Category: Claridge J]] | |||
[[Category: Edwards P]] | |||
[[Category: Libich D]] | |||
[[Category: Loo T]] | |||
[[Category: Norris G]] | |||
[[Category: Pascal S]] | |||
[[Category: Patchett M]] | |||
[[Category: Schwalbe M]] | |||
[[Category: Stepper J]] | |||
[[Category: Venugopal H]] | |||
Latest revision as of 01:08, 21 November 2024
Structure of Glycocin F
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