2x8r: Difference between revisions
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New page: '''Unreleased structure''' The entry 2x8r is ON HOLD until Paper Publication Authors: Korczynska, J.E., Danielsen, S., Schagerlof, U., Turkenburg, J.P., Davies, G.J., Wilson, K.S., Tayl... |
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The | ==The structure of a family GH25 lysozyme from Aspergillus fumigatus== | ||
<StructureSection load='2x8r' size='340' side='right'caption='[[2x8r]], [[Resolution|resolution]] 1.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2x8r]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus_Af293 Aspergillus fumigatus Af293]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X8R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X8R FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x8r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x8r OCA], [https://pdbe.org/2x8r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x8r RCSB], [https://www.ebi.ac.uk/pdbsum/2x8r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x8r ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A4DA29_ASPFU A4DA29_ASPFU] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x8/2x8r_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2x8r ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Lysins are important biomolecules which cleave the bacterial cell-wall polymer peptidoglycan. They are finding increasing commercial and medical application. In order to gain an insight into the mechanism by which these enzymes operate, the X-ray structure of a CAZy family GH25 ;lysozyme' from Aspergillus fumigatus was determined. This is the first fungal structure from the family and reveals a modified alpha/beta-barrel-like fold in which an eight-stranded beta-barrel is flanked by three alpha-helices. The active site lies toward the bottom of a negatively charged pocket and its layout has much in common with other solved members of the GH25 and related GH families. A conserved active-site DXE motif may be implicated in catalysis, lending further weight to the argument that this glycoside hydrolase family operates via a ;substrate-assisted' catalytic mechanism. | |||
The structure of a family GH25 lysozyme from Aspergillus fumigatus.,Korczynska JE, Danielsen S, Schagerlof U, Turkenburg JP, Davies GJ, Wilson KS, Taylor EJ Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Sep 1;66(Pt, 9):973-7. Epub 2010 Aug 21. PMID:20823508<ref>PMID:20823508</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2x8r" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Lysozyme 3D structures|Lysozyme 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Aspergillus fumigatus Af293]] | |||
[[Category: Large Structures]] | |||
[[Category: Danielsen S]] | |||
[[Category: Davies GJ]] | |||
[[Category: Korczynska JE]] | |||
[[Category: Schagerlof U]] | |||
[[Category: Taylor EJ]] | |||
[[Category: Turkenburg JP]] | |||
[[Category: Wilson KS]] | |||
Latest revision as of 07:49, 9 October 2024
The structure of a family GH25 lysozyme from Aspergillus fumigatus
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