3i0q: Difference between revisions

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{{Seed}}
[[Image:3i0q.png|left|200px]]


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==Crystal Structure of the AMP-bound complex of Spectinomycin Phosphotransferase, APH(9)-Ia==
The line below this paragraph, containing "STRUCTURE_3i0q", creates the "Structure Box" on the page.
<StructureSection load='3i0q' size='340' side='right'caption='[[3i0q]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3i0q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila_serogroup_1 Legionella pneumophila serogroup 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I0Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I0Q FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
{{STRUCTURE_3i0q|  PDB=3i0q  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i0q OCA], [https://pdbe.org/3i0q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i0q RCSB], [https://www.ebi.ac.uk/pdbsum/3i0q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i0q ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O06916_LEGPN O06916_LEGPN]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i0/3i0q_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i0q ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Aminoglycoside phosphotransferases (APHs) constitute a diverse group of enzymes that are often the underlying cause of aminoglycoside resistance in the clinical setting. Several APHs have been extensively characterized, including the elucidation of the three-dimensional structure of two APH(3') isozymes and an APH(2'') enzyme. Although many APHs are plasmid-encoded and are capable of inactivating numerous 2-deoxystreptmaine aminoglycosides with multiple regiospecificity, APH(9)-Ia, isolated from Legionella pneumophila, is an unusual enzyme among the APH family for its chromosomal origin and its specificity for a single non-2-deoxystreptamine aminoglycoside substrate, spectinomycin. We describe here the crystal structures of APH(9)-Ia in its apo form, its binary complex with the nucleotide, AMP, and its ternary complex bound with ADP and spectinomycin. The structures reveal that APH(9)-Ia adopts the bilobal protein kinase-fold, analogous to the APH(3') and APH(2'') enzymes. However, APH(9)-Ia differs significantly from the other two types of APH enzymes in its substrate binding area and that it undergoes a conformation change upon ligand binding. Moreover, kinetic assay experiments indicate that APH(9)-Ia has stringent substrate specificity as it is unable to phosphorylate substrates of choline kinase or methylthioribose kinase despite high structural resemblance. The crystal structures of APH(9)-Ia demonstrate and expand our understanding of the diversity of the APH family, which in turn will facilitate the development of new antibiotics and inhibitors.


===Crystal Structure of the AMP-bound complex of Spectinomycin Phosphotransferase, APH(9)-Ia===
Structure of the antibiotic resistance factor spectinomycin phosphotransferase from Legionella pneumophila.,Fong DH, Lemke CT, Hwang J, Xiong B, Berghuis AM J Biol Chem. 2010 Mar 26;285(13):9545-55. Epub 2010 Jan 19. PMID:20089863<ref>PMID:20089863</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3i0q" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_20089863}}, adds the Publication Abstract to the page
*[[Phosphotransferase 3D structures|Phosphotransferase 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 20089863 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_20089863}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
3I0Q is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I0Q OCA].
[[Category: Legionella pneumophila serogroup 1]]
 
[[Category: Berghuis AM]]
==Reference==
[[Category: Fong DH]]
<ref group="xtra">PMID:20089863</ref><references group="xtra"/>
[[Category: Hwang J-Y]]
[[Category: Legionella pneumophila]]
[[Category: Lemke CT]]
[[Category: Berghuis, A M.]]
[[Category: Xiong B]]
[[Category: Fong, D H.]]
[[Category: Hwang, J Y.]]
[[Category: Lemke, C T.]]
[[Category: Xiong, B.]]
[[Category: Aminoglycoside phosphotransferase]]
[[Category: Antibiotic resistance]]
[[Category: Protein kinase]]
[[Category: Transferase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr  7 10:12:56 2010''
Retrieved from "https://proteopedia.org/3i0q"