4sod: Difference between revisions

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{{Theoretical_model}}
{{Theoretical_model}}
{{Seed}}
[[Image:4sod.png|left|200px]]


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==RATIONAL DESIGN AND EXPRESSION OF A HEPARIN-TARGETED HUMAN SUPEROXIDE DISMUTASE==
The line below this paragraph, containing "STRUCTURE_4sod", creates the "Structure Box" on the page.
<StructureSection load='4sod' size='340' side='right'caption='[[4sod]]' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
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<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4SOD FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4sod FirstGlance], [https://www.ebi.ac.uk/pdbsum/4sod PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4sod ProSAT]</span></td></tr>
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</table>
{{STRUCTURE_4sod|  PDB=4sod  |  SCENE=  }}
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In order to improve the therapeutic effectiveness of human Cu,Zn superoxide dismutase (HSOD) by targeting it to cell surfaces and increasing its circulatory half-life, we have designed and expressed a heparin-binding derivative of HSOD. This design was based on the idea that structurally independent protein units, HSOD and the heparin-binding A+ helix from protein C inhibitor, could be combined with a carefully chosen linker, GlyProGly, to form a stable, bifunctional protein. The chimeric HSOD-GlyProGly-A+ protein was expressed and secreted to the periplasm of E. coli and had normal SOD activity. HSOD-GlyProGly-A+ had a significantly increased retention time relative to wild-type HSOD on a heparin affinity column, indicating that it was successfully targeted to heparin, and this binding was maintained at physiological ionic strength. When administered to mice, HSOD-GlyProGly-A+ had a half-life of approximately 15 minutes, twice that of wild-type HSOD. Our rational design approach should be generally applicable to the creation of bifunctional chimeric molecules.


===RATIONAL DESIGN AND EXPRESSION OF A HEPARIN-TARGETED HUMAN SUPEROXIDE DISMUTASE===
Rational design and expression of a heparin-targeted human superoxide dismutase.,Boissinot M, Kuhn LA, Lee P, Fisher CL, Wang Y, Hallewell RA, Tainer JA Biochem Biophys Res Commun. 1993 Jan 15;190(1):250-6. PMID:8422249<ref>PMID:8422249</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 4sod" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 8422249 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_8422249}}
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</StructureSection>
==About this Structure==
[[Category: Theoretical Model]]
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4SOD OCA].
[[Category: Large Structures]]
 
==Reference==
<ref group="xtra">PMID:8422249</ref><references group="xtra"/>
[[Category: Fisher, C L]]
[[Category: Fisher, C L]]
[[Category: Kuhn, L A]]
[[Category: Kuhn, L A]]
[[Category: Tainer, J A]]
[[Category: Tainer, J A]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr  8 08:29:42 2010''
Retrieved from "https://proteopedia.org/4sod"