2kh9: Difference between revisions

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{{Seed}}
[[Image:2kh9.png|left|200px]]


<!--
==Solution structure of yeast Prp24-RRM2 bound to a fragment of U6 RNA==
The line below this paragraph, containing "STRUCTURE_2kh9", creates the "Structure Box" on the page.
<StructureSection load='2kh9' size='340' side='right'caption='[[2kh9]]' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2kh9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KH9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KH9 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kh9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kh9 OCA], [https://pdbe.org/2kh9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kh9 RCSB], [https://www.ebi.ac.uk/pdbsum/2kh9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kh9 ProSAT]</span></td></tr>
{{STRUCTURE_2kh9|  PDB=2kh9  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/PRP24_YEAST PRP24_YEAST] Binds preferentially to the U4/U6 hybrid snRNAs. Can stimulate the annealing of U4 and U6. Could participate in both the formation and disassembly of the U4/U6 hybrid during splicing.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kh/2kh9_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kh9 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
U6 RNA plays a critical role in pre-mRNA splicing. Assembly of U6 into the spliceosome requires a significant structural rearrangement and base-pairing with U4 RNA. In the yeast Saccharomyces cerevisiae, this process requires the essential splicing factor Prp24. We present the characterization and structure of a complex containing one of Prp24's four RNA recognition motif (RRM) domains, RRM2, and a fragment of U6 RNA. NMR methods were used to identify the preferred U6 binding sequence of RRM2 (5'-GAGA-3'), measure the affinity of the interaction, and solve the structure of RRM2 bound to the hexaribonucleotide AGAGAU. Interdomain contacts observed between RRM2 and RRM3 in a crystal structure of the free protein are not detectable in solution. A structural model of RRM1 and RRM2 bound to a longer segment of U6 RNA is presented, and a partial mechanism for Prp24's annealing activity is proposed.


===Solution structure of yeast Prp24-RRM2 bound to a fragment of U6 RNA===
Structure and functional implications of a complex containing a segment of U6 RNA bound by a domain of Prp24.,Martin-Tumasz S, Reiter NJ, Brow DA, Butcher SE RNA. 2010 Apr;16(4):792-804. Epub 2010 Feb 24. PMID:20181740<ref>PMID:20181740</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2kh9" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_20181740}}, adds the Publication Abstract to the page
*[[Pre-mRNA splicing factors 3D structures|Pre-mRNA splicing factors 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 20181740 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_20181740}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
2KH9 is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KH9 OCA].
 
==Reference==
<ref group="xtra">PMID:20181740</ref><references group="xtra"/>
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Butcher, S E.]]
[[Category: Butcher SE]]
[[Category: Martin-Tumasz, S A.]]
[[Category: Martin-Tumasz SA]]
[[Category: Mrna processing]]
[[Category: Mrna splicing]]
[[Category: Nucleus]]
[[Category: Phosphoprotein]]
[[Category: Protein/rna]]
[[Category: Rna-binding]]
[[Category: Rrm]]
[[Category: Snrnp]]
[[Category: Splicing]]
[[Category: Splicing-rna complex]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 14 09:06:32 2010''

Latest revision as of 09:38, 22 May 2024

Solution structure of yeast Prp24-RRM2 bound to a fragment of U6 RNA

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