Disulfide Connectivity of Velaglucerase: Difference between revisions
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<applet load='1ogs' size='[450,338]' frame='true' align='right' | <applet load='1ogs' size='[450,338]' frame='true' align='right' | ||
caption='Human beta-glucocerebrosidase (1ogs)' scene='User:Adriana_Kita/Sandbox_1/Gcase_whole/5'/> | caption='Human beta-glucocerebrosidase ([[1ogs]])' scene='User:Adriana_Kita/Sandbox_1/Gcase_whole/5'/> | ||
Human beta-glucocerebrosidase, also known as glucoceremidase, GCase, and velaglucerase, is an enzyme that is commonly found to be deficient in patients with Gaucher's disease. | Human beta-glucocerebrosidase, also known as glucoceremidase, GCase, and velaglucerase, is an enzyme that is commonly found to be deficient in patients with Gaucher's disease. <ref group="xtra">PMID:12792654</ref><ref group="xtra">PMID:17524049</ref><ref group="xtra">PMID:19741058</ref> | ||
Currently, there are three drugs on the market to treat this disease. One being Genzyme's Cerezyme, Protalix's Taliglucerase and SHIRE's VPRIV. | |||
Currently, there are | |||
The typical method used to sequence current biologics, as well as map post translational modifications, is a combination of proteolytic digestion followed by LC/MS. Unfortunately, sometimes this method is not enough for complete identification of some post translational modifications. I am interested in the oxidation of Cys residues for the formation of disulfide bonds. | The typical method used to sequence current biologics, as well as map post translational modifications, is a combination of proteolytic digestion followed by LC/MS. Unfortunately, sometimes this method is not enough for complete identification of some post translational modifications. I am interested in the oxidation of Cys residues for the formation of disulfide bonds. | ||
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<applet load='1ogs' size='[450,338]' frame='true' align='right' | <applet load='1ogs' size='[450,338]' frame='true' align='right' | ||
caption='Disulfide Connectivity of GCase' scene='User:Adriana_Kita/Sandbox_1/Gcase_disulfide_1/2'/> | caption='Disulfide Connectivity of GCase ([[1ogs]])' scene='User:Adriana_Kita/Sandbox_1/Gcase_disulfide_1/2'/> | ||
=== Disulfide Structure === | === Disulfide Structure === | ||
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'''Disulfide Connectivity:''' ''C4-C16 & C18-C23'' | '''Disulfide Connectivity:''' ''C4-C16 & C18-C23'' | ||
==3D structures of beta-glucocerebrosidase== | |||
[[Acid-beta-glucosidase]] | |||
==Additional Resources== | |||
For additional information, see: [[Metabolic Disorders]] | |||
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==Reference== | ==Reference== | ||
<references group="xtra"/> | |||
[[Category: Glucosylceramidase]] | [[Category: Glucosylceramidase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
