3lyq: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
 
(6 intermediate revisions by the same user not shown)
Line 1: Line 1:
{{Seed}}
[[Image:3lyq.png|left|200px]]


<!--
==Crystal structure of IpgB2 from Shigella flexneri==
The line below this paragraph, containing "STRUCTURE_3lyq", creates the "Structure Box" on the page.
<StructureSection load='3lyq' size='340' side='right'caption='[[3lyq]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3lyq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LYQ FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FEO:MU-OXO-DIIRON'>FEO</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
{{STRUCTURE_3lyq|  PDB=3lyq  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3lyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lyq OCA], [https://pdbe.org/3lyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3lyq RCSB], [https://www.ebi.ac.uk/pdbsum/3lyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3lyq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9AJW7_SHIFL Q9AJW7_SHIFL]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ly/3lyq_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3lyq ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A common theme in bacterial pathogenesis is the manipulation of eukaryotic cells by targeting the cytoskeleton. This is in most cases achieved either by modifying actin, or indirectly via activation of key regulators controlling actin dynamics such as Rho-GTPases. A novel group of bacterial virulence factors termed the WXXXE family has emerged as guanine nucleotide exchange factors (GEFs) for these GTPases. The precise mechanism of nucleotide exchange, however, has remained unclear. Here we report the structure of the WXXXE-protein IpgB2 from Shigella flexneri and its complex with human RhoA. We unambiguously identify IpgB2 as a bacterial RhoA-GEF and dissect the molecular mechanism of GDP release, an essential prerequisite for GTP binding. Our observations uncover that IpgB2 induces conformational changes on RhoA mimicking DbI- but not DOCK family GEFs. We also show that dissociation of the GDP.Mg(2+) complex is preceded by the displacement of the metal ion to the alpha-phosphate of the nucleotide, diminishing its affinity to the GTPase. These data refine our understanding of the mode of action not only of WXXXE GEFs but also of mammalian GEFs of the DH/PH family.


===Crystal structure of IpgB2 from Shigella flexneri===
Structure of Shigella IpgB2 in complex with human RhoA: implications for the mechanism of bacterial guanine nucleotide exchange factor mimicry.,Klink BU, Barden S, Heidler TV, Borchers C, Ladwein M, Stradal TE, Rottner K, Heinz DW J Biol Chem. 2010 May 28;285(22):17197-208. Epub 2010 Apr 2. PMID:20363740<ref>PMID:20363740</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<!--
</div>
The line below this paragraph, {{ABSTRACT_PUBMED_20363740}}, adds the Publication Abstract to the page
<div class="pdbe-citations 3lyq" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 20363740 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_20363740}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
3LYQ is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LYQ OCA].
 
==Reference==
<ref group="xtra">PMID:20363740</ref><references group="xtra"/>
[[Category: Shigella flexneri]]
[[Category: Shigella flexneri]]
[[Category: Barden, S.]]
[[Category: Barden S]]
[[Category: Borchers, C.]]
[[Category: Borchers C]]
[[Category: Heidler, T V.]]
[[Category: Heidler TV]]
[[Category: Heinz, D W.]]
[[Category: Heinz DW]]
[[Category: Klink, B U.]]
[[Category: Klink BU]]
[[Category: Ladwein, M.]]
[[Category: Ladwein M]]
[[Category: Rottner, K.]]
[[Category: Rottner K]]
[[Category: Stradal, T E.B.]]
[[Category: Stradal TEB]]
[[Category: Bacterial gef]]
[[Category: Cytoskeleton dynamic]]
[[Category: Gef]]
[[Category: Ipgb2]]
[[Category: Rhoa-binding protein]]
[[Category: Ttss effector protein]]
[[Category: Wxxxe]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun  2 08:26:01 2010''
Retrieved from "https://proteopedia.org/3lyq"