3now: Difference between revisions
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New page: '''Unreleased structure''' The entry 3now is ON HOLD Authors: Lee, C.F., Hauenstein, A.V., Fleming, J.K., Gasper, W.C., Engelke, V., Banumathi, S., Bernstein, S.I., Huxford, T. Descrip... |
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The | ==UNC-45 from Drosophila melanogaster== | ||
<StructureSection load='3now' size='340' side='right'caption='[[3now]], [[Resolution|resolution]] 2.99Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3now]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NOW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NOW FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.992Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3now FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3now OCA], [https://pdbe.org/3now PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3now RCSB], [https://www.ebi.ac.uk/pdbsum/3now PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3now ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q960B1_DROME Q960B1_DROME] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
UCS proteins, such as UNC-45, influence muscle contraction and other myosin-dependent motile processes. We report the first X-ray crystal structure of a UCS domain-containing protein, the UNC-45 myosin chaperone from Drosophila melanogaster (DmUNC-45). The structure reveals that the central and UCS domains form a contiguous arrangement of 17 consecutive helical layers that arrange themselves into five discrete armadillo repeat subdomains. Small-angle X-ray scattering data suggest that free DmUNC-45 adopts an elongated conformation and exhibits flexibility in solution. Protease sensitivity maps to a conserved loop that contacts the most carboxy-terminal UNC-45 armadillo repeat subdomain. Amino acid conservation across diverse UCS proteins maps to one face of this carboxy-terminal subdomain, and the majority of mutations that affect myosin-dependent cellular activities lie within or around this region. Our crystallographic, biophysical, and biochemical analyses suggest that DmUNC-45 function is afforded by its flexibility and by structural integrity of its UCS domain. | |||
X-ray Crystal Structure of the UCS Domain-Containing UNC-45 Myosin Chaperone from Drosophila melanogaster.,Lee CF, Hauenstein AV, Fleming JK, Gasper WC, Engelke V, Sankaran B, Bernstein SI, Huxford T Structure. 2011 Mar 9;19(3):397-408. PMID:21397190<ref>PMID:21397190</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3now" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Drosophila melanogaster]] | |||
[[Category: Large Structures]] | |||
[[Category: Banumathi S]] | |||
[[Category: Bernstein SI]] | |||
[[Category: Engelke V]] | |||
[[Category: Fleming JK]] | |||
[[Category: Gasper WC]] | |||
[[Category: Hauenstein AV]] | |||
[[Category: Huxford T]] | |||
[[Category: Lee CF]] | |||
Latest revision as of 00:33, 28 December 2023
UNC-45 from Drosophila melanogaster
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