3nrp: Difference between revisions
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New page: '''Unreleased structure''' The entry 3nrp is ON HOLD Authors: Chan, A.C.K., Murphy, M.E.P. Description: Crystal structure of 'as isolated' uropathogenic E. coli strain F11 FetP recombi... |
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The | ==Crystal structure of 'as isolated' uropathogenic E. coli strain F11 FetP recombinantly expressed in the periplasm of E. coli BL21(DE3)== | ||
<StructureSection load='3nrp' size='340' side='right'caption='[[3nrp]], [[Resolution|resolution]] 1.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3nrp]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_F11 Escherichia coli F11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NRP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NRP FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nrp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nrp OCA], [https://pdbe.org/3nrp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nrp RCSB], [https://www.ebi.ac.uk/pdbsum/3nrp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nrp ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In the uropathogenic Escherichia coli strain F11, in silico genome analysis revealed the dicistronic iron uptake operon fetMP, which is under iron-regulated control mediated by the Fur regulator. The expression of fetMP in a mutant strain lacking known iron uptake systems improved growth under iron depletion and increased cellular iron accumulation. FetM is a member of the iron/lead transporter superfamily and is essential for iron uptake by the Fet system. FetP is a periplasmic protein that enhanced iron uptake by FetM. Recombinant FetP bound Cu(II) and the iron analog Mn(II) at distinct sites. The crystal structure of the FetP dimer reveals a copper site in each FetP subunit that adopts two conformations: CuA with a tetrahedral geometry composed of His(44), Met(90), His(97), and His(127), and CuB, a second degenerate octahedral geometry with the addition of Glu(46). The copper ions of each site occupy distinct positions and are separated by approximately 1.3 A. Nearby, a putative additional Cu(I) binding site is proposed as an electron source that may function with CuA/CuB displacement to reduce Fe(III) for transport by FetM. Together, these data indicate that FetMP is an additional iron uptake system composed of a putative iron permease and an iron-scavenging and potentially iron-reducing periplasmic protein. | |||
Characterization of a Dipartite Iron Uptake System from Uropathogenic Escherichia coli Strain F11.,Koch D, Chan AC, Murphy ME, Lilie H, Grass G, Nies DH J Biol Chem. 2011 Jul 15;286(28):25317-30. Epub 2011 May 19. PMID:21596746<ref>PMID:21596746</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3nrp" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Escherichia coli F11]] | |||
[[Category: Large Structures]] | |||
[[Category: Chan ACK]] | |||
[[Category: Murphy MEP]] | |||
