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New page: left|200px<br /> <applet load="108d" size="450" color="white" frame="true" align="right" spinBox="true" caption="108d" /> '''THE SOLUTION STRUCTURE OF A DNA COMPLEX WIT...
 
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[[Image:108d.gif|left|200px]]<br />
<applet load="108d" size="450" color="white" frame="true" align="right" spinBox="true"
caption="108d" />
'''THE SOLUTION STRUCTURE OF A DNA COMPLEX WITH THE FLUORESCENT BIS INTERCALATOR TOTO DETERMINED BY NMR SPECTROSCOPY'''<br />


==Overview==
==THE SOLUTION STRUCTURE OF A DNA COMPLEX WITH THE FLUORESCENT BIS INTERCALATOR TOTO DETERMINED BY NMR SPECTROSCOPY==
We have used two-dimensional 1H NMR spectroscopy to determine the solution, structure of the DNA oligonucleotide d(5'-CGCTAGCG-3')2 complexed with the, bis-intercalating dye, 1,1'-(4,4,8,8-tetramethyl-4,8-diazaundecamethylene)bis[4-(3-methyl -2,3-, dihydrobenzo-1,3-thiazolyl-2-methylidene)qui nolinium] tetraiodide (TOTO)., The determination of the structure was based on total relaxation matrix, analysis of the NOESY cross-peak intensities using the program MARDIGRAS., Improved procedures to consider the experimental "noise" in NOESY spectra, during these calculations have been employed. The NOE-derived distance, restraints were applied in restrained molecular dynamics calculations., Twenty final structures each were generated for the TOTO complex from both, A-form and B-form dsDNA ... [[http://ispc.weizmann.ac.il/pmbin/getpm?7612596 (full description)]]
<StructureSection load='108d' size='340' side='right'caption='[[108d]]' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[108d]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=108D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=108D FirstGlance]. <br>
108D is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/ ]] with TOT as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id= OCA]].
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TOT:1,1-(4,4,8,8-TETRAMETHYL-4,8-DIAZAUNDECAMETHYLENE)-BIS-4-3-METHYL-2,3-DIHYDRO-(BENZO-1,3-THIAZOLE)-2-METHYLIDENE)-QUINOLINIUM'>TOT</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=108d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=108d OCA], [https://pdbe.org/108d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=108d RCSB], [https://www.ebi.ac.uk/pdbsum/108d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=108d ProSAT]</span></td></tr>
Solution structure of a DNA complex with the fluorescent bis-intercalator TOTO determined by NMR spectroscopy., Spielmann HP, Wemmer DE, Jacobsen JP, Biochemistry. 1995 Jul 11;34(27):8542-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7612596 7612596]
</table>
[[Category: Protein complex]]
__TOC__
[[Category: Jacobsen, J.P.]]
</StructureSection>
[[Category: Spielmann, H.P.]]
[[Category: Large Structures]]
[[Category: Wemmer, D.E.]]
[[Category: Jacobsen JP]]
[[Category: TOT]]
[[Category: Spielmann HP]]
[[Category: bis-thiazole orange]]
[[Category: Wemmer DE]]
[[Category: dna]]
[[Category: fluorescent bis intercalator]]
[[Category: nmr]]
[[Category: toto]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 15:38:39 2007''

Latest revision as of 13:21, 13 March 2024

THE SOLUTION STRUCTURE OF A DNA COMPLEX WITH THE FLUORESCENT BIS INTERCALATOR TOTO DETERMINED BY NMR SPECTROSCOPY

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