TBX3: Difference between revisions
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=Introduction= | =Introduction= | ||
TBX3 acts as a repressor and has had its crystal structure solved to 1.7 Å resolution. There are many differences between the solved structure of TBX3 and its homologue, [[Brachyury]]. Secondary structures and quaternary contacts are slightly different. TBX3 independently recognises two halves of a palindromic site whereas Brachyury is stabilised via interactions between the two monomers. Despite this, the T-box domain of TBX3 and the T-box domain of Brachyury can be superimposed with a rmsd of 1.3 Å for 167 atoms. | '''TBX3''' acts as a repressor and has had its crystal structure solved to 1.7 Å resolution. There are many differences between the solved structure of TBX3 and its homologue, [[Brachyury]]. Secondary structures and quaternary contacts are slightly different. TBX3 independently recognises two halves of a palindromic site whereas Brachyury is stabilised via interactions between the two monomers. Despite this, the T-box domain of TBX3 and the T-box domain of Brachyury can be superimposed with a rmsd of 1.3 Å for 167 atoms. See [[Transcription and RNA Processing]]. | ||
=Crystal structure= | =Crystal structure= | ||
{{STRUCTURE_1h6f| PDB=1h6f | | |||
{{STRUCTURE_1h6f| PDB=1h6f | SIZE=400| SCENE= |right|CAPTION=TBX3 dimer complex with DNA and Mg+2 ion, [[1h6f]] }} | |||
TBX3 expressed from residues 101 - 291 were viable proteins and were crystallised in space group P2<sub>1</sub>2<sub>1</sub>2<sub>1</sub>. These included two monomers and one DNA duplex in the asymmetric unit. The monomers themselves can be superimposed to give an rmsd of 0.5 Å. The largest differences between the TBX3 crystal structure and the T-box domain of Brachyury is between residues 173 and 184, and between strands F and G. Four residues are inserted between F and G and this adopts a 3<sub>10</sub> conformation. | TBX3 expressed from residues 101 - 291 were viable proteins and were crystallised in space group P2<sub>1</sub>2<sub>1</sub>2<sub>1</sub>. These included two monomers and one DNA duplex in the asymmetric unit. The monomers themselves can be superimposed to give an rmsd of 0.5 Å. The largest differences between the TBX3 crystal structure and the T-box domain of Brachyury is between residues 173 and 184, and between strands F and G. Four residues are inserted between F and G and this adopts a 3<sub>10</sub> conformation. | ||
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The helix 3<sub>10</sub>C, also present in TBX5, contacts DNA in the minor groove and hence widens it. However, overall the DNA is not bent, as seen in other crystal structures of [[T-box proteins]]. The 3<sub>10</sub> helix interacts with DNA using <scene name='User:Helen_Ginn/310c_277-279-281-283/1'>residues N277 (of the preceding loop), F279, K281 and F283.</scene> (See <scene name='User:Helen_Ginn/310c_277/3'>N277 only</scene>, <scene name='User:Helen_Ginn/310c_279/1'>F279 only</scene>, | The helix 3<sub>10</sub>C, also present in TBX5, contacts DNA in the minor groove and hence widens it. However, overall the DNA is not bent, as seen in other crystal structures of [[T-box proteins]]. The 3<sub>10</sub> helix interacts with DNA using <scene name='User:Helen_Ginn/310c_277-279-281-283/1'>residues N277 (of the preceding loop), F279, K281 and F283.</scene> (See <scene name='User:Helen_Ginn/310c_277/3'>N277 only</scene>, <scene name='User:Helen_Ginn/310c_279/1'>F279 only</scene>, | ||
<scene name='User:Helen_Ginn/310c_281/1'>F281 only</scene> and <scene name='User:Helen_Ginn/310c_283/1'>F283 only</scene>). | <scene name='User:Helen_Ginn/310c_281/1'>F281 only</scene> and <scene name='User:Helen_Ginn/310c_283/1'>F283 only</scene>). | ||
=3D structures of TBX3= | |||
[[T-box proteins]] | |||
=Additional Resources= | |||
For additional information, see: [[Transcription and RNA Processing]] | |||
<br /> | |||
==Reference== | |||
<ref group="xtra">PMID:12005433</ref><references group="xtra"/> | |||
[[Category: Homo sapiens]] | |||
[[Category: Coll, M.]] | |||
[[Category: Muller, C W.]] | |||
[[Category: Developmental protein]] | |||
[[Category: Dna-binding]] | |||
[[Category: Holt-oram-syndrome]] | |||
[[Category: Ig-fold]] | |||
[[Category: Nuclear protein]] | |||
[[Category: Repressor]] | |||
[[Category: T-box transcription factor]] | |||
[[Category: Tbx3]] | |||
[[Category: Ulnar-mammary syndrome]] | |||
