Luciferase: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
Wayne Decatur (talk | contribs)
Editor, widgeteditor
7,824 edits
mNo edit summary
Michal Harel (talk | contribs)
32,838 edits
No edit summary
 
(53 intermediate revisions by 6 users not shown)
Line 1: Line 1:
==See Also==
<StructureSection load='3iep' size='350' side='right' scene='Luciferase/Cv/1' caption='Luciferase (PDB code [[3iep]])'>
*[[1ba3]] firefly luciferase enzyme from ''Photinus pyralis''
== Function ==
*[[2psd]], [[2pse]], [[2psf]], [[2psh]], [[2psj]], and [[2rh7]] the luciferase from ''Renilla reniformis'' (RLuc)
[[Luciferase]] (Luc) or '''luciferin monooxygenase''' (Lucmo)is an oxidative enzyme used in bioluminescence. It catalyzes the oxidation of the pigment luciferin producing oxyluciferin and light<ref>PMID:2030669</ref>.  See detailed analysis in<br />
*[[1bsl]] 1.95&Aring; heterodimer of bacterial luciferase from ''Vibrio harveyi''
* [[Luciola cruciata luciferase]]<br />
*[[1brl]] 2.4&Aring; heterodimer of bacterial luciferase from ''Vibrio harveyi''
* [[Luciferase FMN complex- Vibrio harveyi]]<br />
*[[1xkj]] homodimer of bacterial luciferase from ''Vibrio harveyi''
* [[Dinoflagellate luciferase]]<br />
*[[2d1q]], [[2d1r]] and [[2d1s]] thermostable Japanese Firefly (''Luciola cruciata'') Luciferase complexed with High-energy intermediate analog
* [[Luciferase (Hebrew)]].
*[[2d1t]] thermostable Japanese Firefly Luciferase red-color emission S286N mutant
 
*[[1lci]] firefly luciferase enzyme from ''Photinus pyralis''
See [[Colored & Bioluminescent Protein]].
*[[1luc]] The 1.5&Aring; resolution crystal structure of bacterial luciferase from ''Vibrio harveyi''
 
*[[1vpr]] luciferase of ''Lingulodinium polyedrum'', a marine bioluminescent dinoflagellate
== Relevance ==
*[[3ie3]],[[3iep]] and [[3ier]] Firefly luciferase (FLuc) bound to its natural substrate ATP and the inhibitor PTC124<ref>PMID: 20194791</ref>
Luc is used in biological research to assess gene transfection into cells where the Luc gene is added to the transfected gene and the light emission by Luc measured for indication of the transfection success<ref>PMID:3072883</ref>.  '''NanoLuc luciferase''' is a small mutated luciferase from shrimp with enhanced stability and a significant increase in luminescence <ref>PMID:27045664</ref>.
*[[3fgc]] Bacterial luciferase from ''Vibrio harveyi''<ref>PMID: 19435287</ref>
 
*[[Green Fluorescent Protein]]
== 3D Structures of Luciferase ==
[[Luciferase 3D structures]]
 
</StructureSection>
 
==Additional information==
[[Image:2d1s jena3dviewer image.jpg|left|220px]]
<scene name='Luciferase/2d1s/1'>Luciferase</scene> ([[Luciola cruciata luciferase|2d1s]]) {{Link Toggle FancyCartoonHighQualityView}}
 
 
Several organisms are bioluminescent, meaning that they produce light. Luciferase is one of the enzymes that catalyzes light emitting reactions in organisms.
{{Clear}}
 
* For additional information, see: [[Colored & Bioluminescent Proteins]]


==References==
==References==
{{Reflist}}
{{Reflist}}


==Additional Literature and Resources==
 
==Additional Literature==
<ref group="xtra">PMID: 18949818</ref><ref group="xtra">PMID: 19859663</ref><references group="xtra"/>
<ref group="xtra">PMID: 18949818</ref><ref group="xtra">PMID: 19859663</ref><references group="xtra"/>
==External Resources==
* [http://news.yahoo.com/s/ap/20100911/ap_on_re_us/us_fireflies_military_research Yahoo news coverage of luciferase studies funded research for military applications]
* [http://en.wikipedia.org/wiki/Luciferase Luciferase at Wikipedia]
* [http://en.wikipedia.org/wiki/Luciferase Luciferase at Wikipedia]
* [http://www.pdb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb78_1.html Luciferase: June 2006 Molecule of the Month] as part of the series of tutorials that are at [http://www.pdb.org/pdb/home/home.do the RCSB Protein Data Bank] and written by [[User:David_S._Goodsell|David S. Goodsell]]
* [http://www.pdb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb78_1.html Luciferase: June 2006 Molecule of the Month] as part of the series of tutorials that are at [http://www.pdb.org/pdb/home/home.do the RCSB Protein Data Bank] and written by [[User:David_S._Goodsell|David S. Goodsell]]
[[Category: Photinus pyralis]]
[[Category: Renilla reniformis]]
[[Category: Renilla-luciferin 2-monooxygenase]]
[[Category: Luciola cruciata]]
[[Category: Luciferase]]
[[Category: Atp-binding]]
[[Category: Luminescence]]
[[Category: Magnesium]]
[[Category: Metal-binding]]
[[Category: Monooxygenase]]
[[Category: Nucleotide-binding]]
[[Category: Oxidoreductase]]
[[Category: Peroxisome]]
[[Category: Photoprotein]]
[[Category: Alpha/beta-hydrolase]]
[[Category:Topic Page]]

Latest revision as of 07:42, 11 July 2024

Luciferase (PDB code 3iep)

Drag the structure with the mouse to rotate

Additional information

Luciferase (Luciola cruciata luciferase)


Several organisms are bioluminescent, meaning that they produce light. Luciferase is one of the enzymes that catalyzes light emitting reactions in organisms.

References


Additional Literature

  1. Marques SM, Esteves da Silva JC. Firefly bioluminescence: a mechanistic approach of luciferase catalyzed reactions. IUBMB Life. 2009 Jan;61(1):6-17. PMID:18949818 doi:10.1002/iub.134
  2. Inouye S. Firefly luciferase: an adenylate-forming enzyme for multicatalytic functions. Cell Mol Life Sci. 2010 Feb;67(3):387-404. Epub 2009 Oct 27. PMID:19859663 doi:10.1007/s00018-009-0170-8

External Resources

Retrieved from "https://proteopedia.org/index.php?title=Luciferase&oldid=4189567"