3nt8: Difference between revisions
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New page: '''Unreleased structure''' The entry 3nt8 is ON HOLD until Paper Publication Authors: Asojo, O.A. Description: Crystal Structure of Na-ASP-1 ''Page seeded by [http://oca.weizmann.ac.i... |
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==Crystal Structure of Na-ASP-1== | |||
<StructureSection load='3nt8' size='340' side='right'caption='[[3nt8]], [[Resolution|resolution]] 2.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3nt8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Necator_americanus Necator americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NT8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NT8 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nt8 OCA], [https://pdbe.org/3nt8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nt8 RCSB], [https://www.ebi.ac.uk/pdbsum/3nt8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nt8 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/O76744_NECAM O76744_NECAM] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Major proteins secreted by the infective larval stage hookworms upon host entry include Ancylostoma secreted proteins (ASPs), which are characterized by one or two CAP (cysteine-rich secretory protein/antigen 5/pathogenesis related-1) domains. The CAP domain has been reported in diverse phylogenetically unrelated proteins, but has no confirmed function. The first structure of a two-CAP-domain protein, Na-ASP-1, from the major human hookworm parasite Necator americanus was refined to a resolution limit of 2.2 A. The structure was solved by molecular replacement (MR) using Na-ASP-2, a one-CAP-domain ASP, as the search model. The correct MR solution could only be obtained by truncating the polyalanine model of Na-ASP-2 and removing several loops. The structure reveals two CAP domains linked by an extended loop. Overall, the carboxyl-terminal CAP domain is more similar to Na-ASP-2 than to the amino-terminal CAP domain. A large central cavity extends from the amino-terminal CAP domain to the carboxyl-terminal CAP domain, encompassing the putative CAP-binding cavity. The putative CAP-binding cavity is a characteristic cavity in the carboxyl-terminal CAP domain that contains a His and Glu pair. These residues are conserved in all single-CAP-domain proteins, but are absent in the amino-terminal CAP domain. The conserved His residues are oriented such that they appear to be capable of directly coordinating a zinc ion as observed for CAP proteins from reptile venoms. This first structure of a two-CAP-domain ASP can serve as a template for homology modeling of other two-CAP-domain proteins. | |||
Structure of a two-CAP-domain protein from the human hookworm parasite Necator americanus.,Asojo OA Acta Crystallogr D Biol Crystallogr. 2011 May;67(Pt 5):455-62. Epub 2011, Apr 7. PMID:21543848<ref>PMID:21543848</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3nt8" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Necator americanus]] | |||
[[Category: Asojo OA]] | |||
