3nq9: Difference between revisions

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{{Seed}}
[[Image:3nq9.jpg|left|200px]]


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==Bovine beta-lactoglobulin complex with caprylic acid==
The line below this paragraph, containing "STRUCTURE_3nq9", creates the "Structure Box" on the page.
<StructureSection load='3nq9' size='340' side='right'caption='[[3nq9]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3nq9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NQ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NQ9 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=OCA:OCTANOIC+ACID+(CAPRYLIC+ACID)'>OCA</scene></td></tr>
{{STRUCTURE_3nq9|  PDB=3nq9  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nq9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nq9 OCA], [https://pdbe.org/3nq9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nq9 RCSB], [https://www.ebi.ac.uk/pdbsum/3nq9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nq9 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nq/3nq9_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3nq9 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Lactoglobulin is a natural protein present in bovine milk and common component of human diet, known for binding with high affinity wide range of hydrophobic compounds, among them fatty acids 12-20 carbon atoms long. Shorter fatty acids were reported as not binding to beta-lactoglobulin. We used X-ray crystallography and fluorescence spectroscopy to show that lactoglobulin binds also 8- and 10-carbon caprylic and capric acids, however with lower affinity. The determined apparent association constant for lactoglobulin complex with caprylic acid is 10.8 +/- 1.7 x 10(3) M(-1) , while for capric acid is 6.0 +/- 0.5 x 10(3) M(-1) . In crystal structures determined with resolution 1.9 A the caprylic acid is bound in upper part of central calyx near polar residues located at CD loop, while the capric acid is buried deeper in the calyx bottom and does not interact with polar residues at CD loop. In both structures, water molecule hydrogen-bonded to carboxyl group of fatty acid is observed. Different location of ligands in the binding site indicates that competition between polar and hydrophobic interactions is an important factor determining position of the ligand in beta-barrel. Copyright (c) 2010 John Wiley &amp; Sons, Ltd.


===Bovine beta-lactoglobulin complex with caprylic acid===
Two modes of fatty acid binding to bovine beta-lactoglobulin-crystallographic and spectroscopic studies.,Loch J, Polit A, Gorecki A, Bonarek P, Kurpiewska K, Dziedzicka-Wasylewska M, Lewinski K J Mol Recognit. 2011 Mar;24(2):341-9. doi: 10.1002/jmr.1084. Epub 2010 Dec, 14. PMID:21360616<ref>PMID:21360616</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3nq9" style="background-color:#fffaf0;"></div>


==About this Structure==
==See Also==
3NQ9 is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NQ9 OCA].
*[[Beta-lactoglobulin 3D structures|Beta-lactoglobulin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Lewinski, K.]]
[[Category: Large Structures]]
[[Category: Loch, J.]]
[[Category: Lewinski K]]
[[Category: Beta-lactoglobulin]]
[[Category: Loch J]]
[[Category: Bovine milk]]
[[Category: Caprylic acid]]
[[Category: Fatty acid]]
[[Category: Lipocalin]]
[[Category: Octanoic acid]]
[[Category: Transport protein]]
 
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Retrieved from "https://proteopedia.org/3nq9"