3ofw: Difference between revisions
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New page: '''Unreleased structure''' The entry 3ofw is ON HOLD Authors: Garcia-Fernandez, R., Redecke, L., Pons, T., Perbandt, M., Talavera, A., Gil, D., Gonzalez, Y., de los Angeles Chavez, M., ... |
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The | ==Crystal structure of recombinant Kunitz Type serine protease Inhibitor-1 from the Carribean sea anemone stichodactyla helianthus== | ||
<StructureSection load='3ofw' size='340' side='right'caption='[[3ofw]], [[Resolution|resolution]] 2.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3ofw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Stichodactyla_helianthus Stichodactyla helianthus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OFW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OFW FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ofw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ofw OCA], [https://pdbe.org/3ofw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ofw RCSB], [https://www.ebi.ac.uk/pdbsum/3ofw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ofw ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/VKT1_STIHL VKT1_STIHL] Active against serine, cysteine, and aspartic proteases. Can bind vertebrate trypsin and chymotrypsin.<ref>PMID:22975140</ref> <ref>PMID:9027993</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The BPTI/Kunitz-type inhibitor family includes several extremely potent serine protease inhibitors. To date, the inhibitory mechanisms have only been studied for mammalian inhibitors. Here, the first crystal structure of a BPTI/Kunitz-type inhibitor from a marine invertebrate (rShPI-1A) is reported to 2.5 A resolution. Crystallization of recombinant rShPI-1A required the salt-induced dissociation of a trypsin complex that was previously formed to avoid intrinsic inhibitor aggregates in solution. The rShPI-1A structure is similar to the NMR structure of the molecule purified from the natural source, but allowed the assignment of disulfide-bridge chiralities and the detection of an internal stabilizing water network. A structural comparison with other BPTI/Kunitz-type canonical inhibitors revealed unusual varphi angles at positions 17 and 30 to be a particular characteristic of the family. A significant clustering of varphi and psi angle values in the glycine-rich remote fragment near the secondary binding loop was additionally identified, but its impact on the specificity of rShPI-1A and similar molecules requires further study. | |||
Structure of the recombinant BPTI/Kunitz-type inhibitor rShPI-1A from the marine invertebrate Stichodactyla helianthus.,Garcia-Fernandez R, Pons T, Meyer A, Perbandt M, Gonzalez-Gonzalez Y, Gil D, de los Angeles Chavez M, Betzel C, Redecke L Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Nov 1;68(Pt 11):1289-93., doi: 10.1107/S1744309112039085. Epub 2012 Oct 26. PMID:23143234<ref>PMID:23143234</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3ofw" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Stichodactyla helianthus]] | |||
[[Category: Betzel C]] | |||
[[Category: Garcia-Fernandez R]] | |||
[[Category: Gil D]] | |||
[[Category: Gonzalez Y]] | |||
[[Category: Perbandt M]] | |||
[[Category: Pons T]] | |||
[[Category: Redecke L]] | |||
[[Category: Talavera A]] | |||
[[Category: De los Angeles Chavez M]] | |||
