3n5a: Difference between revisions

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{{Seed}}
[[Image:3n5a.jpg|left|200px]]


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==Synaptotagmin-7, C2B-domain, calcium bound==
The line below this paragraph, containing "STRUCTURE_3n5a", creates the "Structure Box" on the page.
<StructureSection load='3n5a' size='340' side='right'caption='[[3n5a]], [[Resolution|resolution]] 1.44&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3n5a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N5A FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.441&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
{{STRUCTURE_3n5a|  PDB=3n5a  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n5a OCA], [https://pdbe.org/3n5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n5a RCSB], [https://www.ebi.ac.uk/pdbsum/3n5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n5a ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SYT7_MOUSE SYT7_MOUSE] May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n5/3n5a_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3n5a ConSurf].
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== Publication Abstract from PubMed ==
Synaptotagmins are known to mediate diverse forms of Ca2+-triggered exocytosis through their C2 domains, but the principles underlying functional differentiation among them are unclear. Synaptotagmin-1 functions as a Ca2+ sensor in neurotransmitter release at central nervous system synapses, but synaptotagmin-7 does not, and yet both isoforms act as Ca2+ sensors in chromaffin cells. To shed light into this apparent paradox, we have performed rescue experiments in neurons from synaptotagmin-1 knockout mice using a chimera that contains the synaptotagmin-1 sequence with its C2B domain replaced by the synaptotagmin-7 C2B domain (Syt1/7). Rescue was not achieved either with the WT Syt1/7 chimera or with nine mutants where residues that are distinct in synaptotagmin-7 were restored to those present in synaptotagmin-1. To investigate whether these results arise because of unique conformational features of the synaptotagmin-7 C2B domain, we determined its crystal structure at 1.44 A resolution. The synaptotagmin-7 C2B domain structure is very similar to that of the synaptotagmin-1 C2B domain and contains three Ca2+-binding sites. Two of the Ca2+-binding sites of the synaptotagmin-7 C2B domain are also present in the synaptotagmin-1 C2B domain and have analogous ligands to those determined for the latter by NMR spectroscopy, suggesting that a discrepancy observed in a crystal structure of the synaptotagmin-1 C2B domain arose from crystal contacts. Overall, our results suggest that functional differentiation in synaptotagmins arises in part from subtle sequence changes that yield dramatic functional differences.


===Synaptotagmin-7, C2B-domain, calcium bound===
Structural and mutational analysis of functional differentiation between synaptotagmins-1 and -7.,Xue M, Craig TK, Shin OH, Li L, Brautigam CA, Tomchick DR, Sudhof TC, Rosenmund C, Rizo J PLoS One. 2010 Sep 2;5(9). pii: e12544. PMID:20824061<ref>PMID:20824061</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3n5a" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_20824061}}, adds the Publication Abstract to the page
*[[Synaptotagmin 3D structures|Synaptotagmin 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 20824061 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_20824061}}
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</StructureSection>
==About this Structure==
[[Category: Large Structures]]
3N5A is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5A OCA].
 
==Reference==
<ref group="xtra">PMID:20824061</ref><references group="xtra"/>
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Craig, T K.]]
[[Category: Craig TK]]
[[Category: Rizo, J.]]
[[Category: Rizo J]]
[[Category: Tomchick, D R.]]
[[Category: Tomchick DR]]
[[Category: Calcium/phospholipid binding protein]]
[[Category: Protein transport]]
 
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Retrieved from "https://proteopedia.org/3n5a"