2xsc: Difference between revisions
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New page: '''Unreleased structure''' The entry 2xsc is ON HOLD Authors: Stein, P.E., Boodhoo, A., Tyrrell, G.J., Brunton, J.L., Oeffner, R.D., Bunkoczi, G., Read, R.J. Description: Crystal struc... |
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==Crystal structure of the cell-binding B oligomer of verotoxin-1 from E. coli== | |||
<StructureSection load='2xsc' size='340' side='right'caption='[[2xsc]], [[Resolution|resolution]] 2.05Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2xsc]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1bov 1bov]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XSC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XSC FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.052Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xsc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xsc OCA], [https://pdbe.org/2xsc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xsc RCSB], [https://www.ebi.ac.uk/pdbsum/2xsc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xsc ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/STXB_BPH19 STXB_BPH19] The B subunit is responsible for the binding of the holotoxin to specific receptors on the target cell surface, such as globotriaosylceramide (Gb3) in human intestinal microvilli. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The Shiga toxin family, a group of cytotoxins associated with diarrhoeal diseases and the haemolytic uraemic syndrome, includes Shiga toxin from Shigella dysenteriae type 1 and verotoxins produced by enteropathogenic Escherichia coli. The family belongs to the A-B class of bacterial toxins, which includes the cholera toxin family, pertussis and diphtheria toxins. These toxins all have bipartite structures consisting of an enzymatic A subunit associated with a B oligomer which binds to specific cell-surface receptors, but their amino-acid sequences and pathogenic mechanisms differ. We have determined the crystal structure of the B oligomer of verotoxin-1 from E. coli. The structure unexpectedly resembles that of the B oligomer of the cholera toxin-like heat-labile enterotoxin from E. coli, despite the absence of detectable sequence similarity between these two proteins. This result implies a distant evolutionary relationship between the Shiga toxin and cholera toxin families. We suggest that the cell surface receptor-binding site lies in a cleft between adjacent subunits of the B pentamer, providing a potential target for drugs and vaccines to prevent toxin binding and effect. | |||
Crystal structure of the cell-binding B oligomer of verotoxin-1 from E. coli.,Stein PE, Boodhoo A, Tyrrell GJ, Brunton JL, Read RJ Nature. 1992 Feb 20;355(6362):748-50. PMID:1741063<ref>PMID:1741063</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2xsc" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Shiga toxin|Shiga toxin]] | |||
*[[Shiga toxin 3D structures|Shiga toxin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Escherichia coli]] | |||
[[Category: Large Structures]] | |||
[[Category: Boodhoo A]] | |||
[[Category: Brunton JL]] | |||
[[Category: Bunkoczi G]] | |||
[[Category: Oeffner RD]] | |||
[[Category: Read RJ]] | |||
[[Category: Stein PE]] | |||
[[Category: Tyrrell GJ]] | |||
Latest revision as of 13:59, 1 February 2024
Crystal structure of the cell-binding B oligomer of verotoxin-1 from E. coli
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