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New page: left|200px<br /><applet load="2ecu" size="350" color="white" frame="true" align="right" spinBox="true" caption="2ecu, resolution 1.30Å" /> '''Crystal structure of...
 
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[[Image:2ecu.jpg|left|200px]]<br /><applet load="2ecu" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2ecu, resolution 1.30&Aring;" />
'''Crystal structure of flavin reductase component (HpaC) of 4-hydroxyphenylacetate 3-monooxygenase'''<br />


==Overview==
==Crystal structure of flavin reductase component (HpaC) of 4-hydroxyphenylacetate 3-monooxygenase==
The two-component enzyme, 4-hydroxyphenylacetate 3-monooxygenase, catalyzes the conversion of 4-hydroxyphenylacetate to, 3,4-dihydroxyphenylacetate. In the overall reaction, the oxygenase, component (HpaB) introduces a hydroxyl group into the benzene ring of, 4-hydroxyphenylacetate using molecular oxygen and reduced flavin, while, the reductase component (HpaC) provides free reduced flavins for HpaB. The, crystal structures of HpaC from Thermus thermophilus HB8 in the, ligand-free form, the FAD-containing form, and the ternary complex with, FAD and NAD(+) were determined. In the ligand-free form, two large grooves, are present at the dimer interface, and are occupied by water molecules. A, structural analysis of HpaC containing FAD revealed that FAD has a low, occupancy, indicating that it is not tightly bound to HpaC. This was, further confirmed in flavin dissociation experiments, showing that FAD can, be released from HpaC. The structure of the ternary complex revealed that, FAD and NAD(+) are bound in the groove in the extended and folded, conformation, respectively. The nicotinamide ring of NAD(+) is sandwiched, between the adenine ring of NAD(+) and the isoalloxazine ring of FAD. The, distance between N5 of the isoalloxazine ring and C4 of the nicotinamide, ring is about 3.3 A, sufficient to permit hydride transfer. The structures, of these three states are essentially identical, however, the side chains, of several residues show small conformational changes, indicating an, induced fit upon binding of NADH. Inactivity with respect to NADPH can be, explained as instability of the binding of NADPH with the negatively, charged 2'-phosphate group buried inside the complex, as well as a, possible repulsive effect by the dipole of helix alpha1. A comparison of, the binding mode of FAD with that in PheA2 from Bacillus, thermoglucosidasius A7, which contains FAD as a prosthetic group, reveals, remarkable conformational differences in a less conserved loop region, (Gly83-Gly94) involved in the binding of the AMP moiety of FAD. These data, suggest that variations in the affinities for FAD in the reductases of the, two-component flavin-diffusible monooxygenase family may be attributed to, difference in the interaction between the AMP moiety of FAD and the less, conserved loop region which possibly shows structural divergence. Proteins, 2008. (c) 2007 Wiley-Liss, Inc.
<StructureSection load='2ecu' size='340' side='right'caption='[[2ecu]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2ecu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ECU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ECU FirstGlance]. <br>
2ECU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=1PG:'>1PG</scene> and <scene name='pdbligand=12P:'>12P</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ECU OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=12P:DODECAETHYLENE+GLYCOL'>12P</scene>, <scene name='pdbligand=1PG:2-(2-{2-[2-(2-METHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHANOL'>1PG</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ecu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ecu OCA], [https://pdbe.org/2ecu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ecu RCSB], [https://www.ebi.ac.uk/pdbsum/2ecu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ecu ProSAT], [https://www.topsan.org/Proteins/RSGI/2ecu TOPSAN]</span></td></tr>
Crystal structure of the flavin reductase component (HpaC) of 4-hydroxyphenylacetate 3-monooxygenase from Thermus thermophilus HB8: Structural basis for the flavin affinity., Kim SH, Hisano T, Iwasaki W, Ebihara A, Miki K, Proteins. 2007 Aug 29;70(3):718-730. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17729270 17729270]
</table>
[[Category: Single protein]]
== Function ==
[[Category: Thermus thermophilus]]
[https://www.uniprot.org/uniprot/HPAC_THET8 HPAC_THET8] Catalyzes the reduction of free flavins (FMN, FAD and riboflavin) by NADH. Subsequently, the reduced flavins diffuse to the large HpaB component.<ref>PMID:17729270</ref>
[[Category: Ebihara, A.]]
== Evolutionary Conservation ==
[[Category: Hisano, T.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Iwasaki, W.]]
Check<jmol>
[[Category: Kim, S.H.]]
  <jmolCheckbox>
[[Category: Miki, K.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ec/2ecu_consurf.spt"</scriptWhenChecked>
[[Category: 12P]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: 1PG]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: flavin diffusible]]
  </jmolCheckbox>
[[Category: flavin reductase]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ecu ConSurf].
[[Category: oxidoreductase]]
<div style="clear:both"></div>
[[Category: two-component monooxygenase]]
== References ==
[[Category: x-ray diffraction]]
<references/>
 
__TOC__
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:05:28 2008''
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermus thermophilus HB8]]
[[Category: Ebihara A]]
[[Category: Hisano T]]
[[Category: Iwasaki W]]
[[Category: Kim SH]]
[[Category: Miki K]]

Latest revision as of 06:25, 3 April 2024

Crystal structure of flavin reductase component (HpaC) of 4-hydroxyphenylacetate 3-monooxygenase

2ecu, resolution 1.30Å

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