2xl1: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,332,843 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,332,843 edits
No edit summary
 
(8 intermediate revisions by the same user not shown)
Line 1: Line 1:
{{Seed}}
[[Image:2xl1.jpg|left|200px]]


<!--
==Structural basis of translational stalling by human cytomegalovirus (hCMV) and fungal arginine attenuator peptide (AAP)==
The line below this paragraph, containing "STRUCTURE_2xl1", creates the "Structure Box" on the page.
<StructureSection load='2xl1' size='340' side='right'caption='[[2xl1]]' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2xl1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XL1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XL1 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-->
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xl1 OCA], [https://pdbe.org/2xl1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xl1 RCSB], [https://www.ebi.ac.uk/pdbsum/2xl1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xl1 ProSAT]</span></td></tr>
{{STRUCTURE_2xl1|  PDB=2xl1  |  SCENE= }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/AAP_NEUCR AAP_NEUCR] Arginine attenuator peptide (AAP) that has a regulatory role in the production of arginine-specific carbamoyl phosphate synthetase. Encoded by an upstream open reading frame (uORF) within the 5'-leader region of arginine-specific carbamoyl phosphate synthetase small chain (arg-2) mRNA, it attenuates the translation of the downstream arg-2 ORF. In the presence of high concentrations of arginine, ribosomes translating the uORF encoding AAP stall at the termination codon, resulting in reduced translation from the downstream arg-2 initiation codon.<ref>PMID:10608810</ref> <ref>PMID:8636015</ref> <ref>PMID:9271370</ref> <ref>PMID:9819438</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Specific regulatory nascent chains establish direct interactions with the ribosomal tunnel, leading to translational stalling. Despite a wealth of biochemical data, structural insight into the mechanism of translational stalling in eukaryotes is still lacking. Here we use cryo-electron microscopy to visualize eukaryotic ribosomes stalled during the translation of two diverse regulatory peptides: the fungal arginine attenuator peptide (AAP) and the human cytomegalovirus (hCMV) gp48 upstream open reading frame 2 (uORF2). The C terminus of the AAP appears to be compacted adjacent to the peptidyl transferase center (PTC). Both nascent chains interact with ribosomal proteins L4 and L17 at tunnel constriction in a distinct fashion. Significant changes at the PTC were observed: the eukaryotic-specific loop of ribosomal protein L10e establishes direct contact with the CCA end of the peptidyl-tRNA (P-tRNA), which may be critical for silencing of the PTC during translational stalling. Our findings provide direct structural insight into two distinct eukaryotic stalling processes.


===STRUCTURAL BASIS OF TRANSLATIONAL STALLING BY HUMAN CYTOMEGALOVIRUS (HCMV) AND FUNGAL ARGININE ATTENUATOR PEPTIDE (AAP)===
Structural basis for translational stalling by human cytomegalovirus and fungal arginine attenuator peptide.,Bhushan S, Meyer H, Starosta AL, Becker T, Mielke T, Berninghausen O, Sattler M, Wilson DN, Beckmann R Mol Cell. 2010 Oct 8;40(1):138-46. PMID:20932481<ref>PMID:20932481</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<!--
</div>
The line below this paragraph, {{ABSTRACT_PUBMED_20932481}}, adds the Publication Abstract to the page
<div class="pdbe-citations 2xl1" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 20932481 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_20932481}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
2XL1 is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XL1 OCA].
[[Category: Neurospora crassa]]
 
[[Category: Meyer NH]]
==Reference==
[[Category: Sattler M]]
<ref group="xtra">PMID:20932481</ref><references group="xtra"/>
[[Category: Meyer, N H.]]
[[Category: Sattler, M.]]
[[Category: Antibiotic]]
[[Category: Cytomegalovirus]]
[[Category: Ribosome]]
[[Category: Translation]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 20 06:35:16 2010''
Retrieved from "https://proteopedia.org/2xl1"