3g37: Difference between revisions

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{{Seed}}
[[Image:3g37.jpg|left|200px]]


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==Cryo-EM structure of actin filament in the presence of phosphate==
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<SX load='3g37' size='340' side='right' viewer='molstar' caption='[[3g37]], [[Resolution|resolution]] 6.00&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3g37]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3G37 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3G37 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
{{STRUCTURE_3g37|  PDB=3g37  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3g37 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3g37 OCA], [https://pdbe.org/3g37 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3g37 RCSB], [https://www.ebi.ac.uk/pdbsum/3g37 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3g37 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ACTS_RABIT ACTS_RABIT] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Assembled actin filaments support cellular signaling, intracellular trafficking, and cytokinesis. ATP hydrolysis triggered by actin assembly provides the structural cues for filament turnover in vivo. Here, we present the cryo-electron microscopic (cryo-EM) structure of filamentous actin (F-actin) in the presence of phosphate, with the visualization of some alpha-helical backbones and large side chains. A complete atomic model based on the EM map identified intermolecular interactions mediated by bound magnesium and phosphate ions. Comparison of the F-actin model with G-actin monomer crystal structures reveals a critical role for bending of the conserved proline-rich loop in triggering phosphate release following ATP hydrolysis. Crystal structures of G-actin show that mutations in this loop trap the catalytic site in two intermediate states of the ATPase cycle. The combined structural information allows us to propose a detailed molecular mechanism for the biochemical events, including actin polymerization and ATPase activation, critical for actin filament dynamics.


===Cryo-EM structure of actin filament in the presence of phosphate===
Structural basis for actin assembly, activation of ATP hydrolysis, and delayed phosphate release.,Murakami K, Yasunaga T, Noguchi TQ, Gomibuchi Y, Ngo KX, Uyeda TQ, Wakabayashi T Cell. 2010 Oct 15;143(2):275-87. PMID:20946985<ref>PMID:20946985</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3g37" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_20946985}}, adds the Publication Abstract to the page
*[[Actin 3D structures|Actin 3D structures]]
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== References ==
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<references/>
{{ABSTRACT_PUBMED_20946985}}
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</SX>
==About this Structure==
[[Category: Large Structures]]
3G37 is a 12 chains structure with sequences from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3G37 OCA].
 
==Reference==
<ref group="xtra">PMID:20946985</ref><references group="xtra"/>
[[Category: Oryctolagus cuniculus]]
[[Category: Oryctolagus cuniculus]]
[[Category: Murakami, K.]]
[[Category: Murakami K]]
[[Category: Noguchi, T Q.]]
[[Category: Noguchi TQ]]
[[Category: Uyeda, T Q.]]
[[Category: Uyeda TQ]]
[[Category: Wakabayshi, T.]]
[[Category: Wakabayshi T]]
[[Category: Yasunaga, T.]]
[[Category: Yasunaga T]]
[[Category: Actin]]
[[Category: Atp-binding]]
[[Category: Cell adhesion]]
[[Category: Cellular signaling]]
[[Category: Contractile protein]]
[[Category: Cryo-em]]
[[Category: Cytokinesis]]
[[Category: Cytoskeleton]]
[[Category: Methylation]]
[[Category: Muscle]]
[[Category: Muscle protein]]
[[Category: Nucleotide-binding]]
[[Category: Phosphoprotein]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov  3 11:07:45 2010''
Retrieved from "https://proteopedia.org/3g37"