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New page: left|200px<br /><applet load="2pt7" size="350" color="white" frame="true" align="right" spinBox="true" caption="2pt7, resolution 2.40Å" /> '''Crystal structure of...
 
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[[Image:2pt7.jpg|left|200px]]<br /><applet load="2pt7" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2pt7, resolution 2.40&Aring;" />
'''Crystal structure of Cag VirB11 (HP0525) and an inhibitory protein (HP1451)'''<br />


==Overview==
==Crystal structure of Cag VirB11 (HP0525) and an inhibitory protein (HP1451)==
Helicobacter pylori is one of the world's most successful human pathogens, causing gastric ulcers and cancers. A key virulence factor of H. pylori is, the Cag pathogenicity island, which encodes a type IV secretion system., HP0525 is an essential component of the Cag system and acts as an inner, membrane associated ATPase. HP0525 forms double hexameric ring structures, with the C-terminal domains (CTDs) forming a closed ring and the, N-terminal domains (NTDs) forming a dynamic, open ring. Here, the crystal, structure of HP0525 in complex with a fragment of HP1451, a protein of, previously unknown function, is reported. The HP1451 construct consists of, two domains similar to nucleic acid-binding domains. Two HP1451 molecules, bind to the HP0525 NTDs on opposite sides of the hexamer, locking it in, the closed form and forming a partial lid over the HP0525 chamber. From, the structure, it is suggested that HP1451 acts as an inhibitory factor of, HP0525 to regulate Cag-mediated secretion, a suggestion confirmed by, results of in vitro ATPase assay and in vivo pull-down experiments.
<StructureSection load='2pt7' size='340' side='right'caption='[[2pt7]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2pt7]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PT7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PT7 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pt7 OCA], [https://pdbe.org/2pt7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pt7 RCSB], [https://www.ebi.ac.uk/pdbsum/2pt7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pt7 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O25990_HELPY O25990_HELPY]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pt/2pt7_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pt7 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Helicobacter pylori is one of the world's most successful human pathogens causing gastric ulcers and cancers. A key virulence factor of H. pylori is the Cag pathogenicity island, which encodes a type IV secretion system. HP0525 is an essential component of the Cag system and acts as an inner membrane associated ATPase. HP0525 forms double hexameric ring structures, with the C-terminal domains (CTDs) forming a closed ring and the N-terminal domains (NTDs) forming a dynamic, open ring. Here, the crystal structure of HP0525 in complex with a fragment of HP1451, a protein of previously unknown function, is reported. The HP1451 construct consists of two domains similar to nucleic acid-binding domains. Two HP1451 molecules bind to the HP0525 NTDs on opposite sides of the hexamer, locking it in the closed form and forming a partial lid over the HP0525 chamber. From the structure, it is suggested that HP1451 acts as an inhibitory factor of HP0525 to regulate Cag-mediated secretion, a suggestion confirmed by results of in vitro ATPase assay and in vivo pull-down experiments.


==About this Structure==
Identification, structure and mode of action of a new regulator of the Helicobacter pylori HP0525 ATPase.,Hare S, Fischer W, Williams R, Terradot L, Bayliss R, Haas R, Waksman G EMBO J. 2007 Nov 28;26(23):4926-34. Epub 2007 Nov 1. PMID:17972918<ref>PMID:17972918</ref>
2PT7 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PT7 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Identification, structure and mode of action of a new regulator of the Helicobacter pylori HP0525 ATPase., Hare S, Fischer W, Williams R, Terradot L, Bayliss R, Haas R, Waksman G, EMBO J. 2007 Nov 28;26(23):4926-34. Epub 2007 Nov 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17972918 17972918]
</div>
[[Category: Helicobacter pylori]]
<div class="pdbe-citations 2pt7" style="background-color:#fffaf0;"></div>
[[Category: Protein complex]]
== References ==
[[Category: Bayliss, R.]]
<references/>
[[Category: Fischer, W.]]
__TOC__
[[Category: Haas, R.]]
</StructureSection>
[[Category: Hare, S.]]
[[Category: Helicobacter pylori 26695]]
[[Category: Terradot, L.]]
[[Category: Large Structures]]
[[Category: Waksman, G.]]
[[Category: Bayliss R]]
[[Category: Williams, R.]]
[[Category: Fischer W]]
[[Category: atpase]]
[[Category: Haas R]]
[[Category: hydrolase/protein binding complex]]
[[Category: Hare S]]
[[Category: protein-protein complex]]
[[Category: Terradot L]]
[[Category: type iv secretion]]
[[Category: Waksman G]]
 
[[Category: Williams R]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:40:11 2008''
Retrieved from "https://proteopedia.org/2pt7"