2l5j: Difference between revisions
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The | ==structure of the spliceosomal phosphopeptide P140 (phosphorylated form)== | ||
<StructureSection load='2l5j' size='340' side='right'caption='[[2l5j]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2l5j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L5J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L5J FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 8 models</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l5j OCA], [https://pdbe.org/2l5j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l5j RCSB], [https://www.ebi.ac.uk/pdbsum/2l5j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l5j ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/RU17_HUMAN RU17_HUMAN] Mediates the splicing of pre-mRNA by binding to the loop I region of U1-snRNA. The truncated isoforms cannot bind U1-snRNA. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The phosphopeptide P140 issued from the spliceosomal U1-70K snRNP protein is recognized by lupus CD4(+) T cells, transiently abolishes T cell reactivity to other spliceosomal peptides in P140-treated MRL/lpr mice, and ameliorates their clinical features. P140 modulates lupus patients' T cell response ex vivo and is currently included in phase IIb clinical trials. Its underlying mechanism of action remains elusive. Here we show that P140 peptide binds a unique cell-surface receptor, the constitutively-expressed chaperone HSC70 protein, known as a presenting-protein. P140 induces apoptosis of activated MRL/lpr CD4(+) T cells. In P140-treated mice, it increases peripheral blood lymphocyte apoptosis and decreases B cell, activated T cell, and CD4(-)CD8(-)B220(+) T cell counts via a specific mechanism strictly depending on gammadelta T cells. Expression of inflammation-linked genes is rapidly regulated in CD4(+) T cells. This work led us to identify a powerful pathway taken by a newly-designed therapeutic peptide to immunomodulate lupus autoimmunity. | |||
The spliceosomal phosphopeptide P140 controls the lupus disease by interacting with the HSC70 protein and via a mechanism mediated by gammadelta T cells.,Page N, Schall N, Strub JM, Quinternet M, Chaloin O, Decossas M, Cung MT, Van Dorsselaer A, Briand JP, Muller S PLoS One. 2009;4(4):e5273. Epub 2009 Apr 23. PMID:19390596<ref>PMID:19390596</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2l5j" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Briand J]] | |||
[[Category: Chaloin O]] | |||
[[Category: Cung M]] | |||
[[Category: Decossas M]] | |||
[[Category: Muller S]] | |||
[[Category: Page N]] | |||
[[Category: Quinternet M]] | |||
[[Category: Schall N]] | |||
[[Category: Strub J]] | |||
[[Category: Van Dorsselaer A]] | |||
