Luciferase: Difference between revisions
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<StructureSection load='3iep' size='350' side='right' scene='Luciferase/Cv/1' caption='Luciferase (PDB code [[3iep]])'> | |||
== Function == | |||
[[Luciferase]] (Luc) or '''luciferin monooxygenase''' (Lucmo)is an oxidative enzyme used in bioluminescence. It catalyzes the oxidation of the pigment luciferin producing oxyluciferin and light<ref>PMID:2030669</ref>. See detailed analysis in<br /> | |||
* [[Luciola cruciata luciferase]]<br /> | |||
* [[Luciferase FMN complex- Vibrio harveyi]]<br /> | |||
* [[Dinoflagellate luciferase]]<br /> | |||
* [[Luciferase (Hebrew)]]. | |||
See [[Colored & Bioluminescent Protein]]. | |||
== Relevance == | |||
Luc is used in biological research to assess gene transfection into cells where the Luc gene is added to the transfected gene and the light emission by Luc measured for indication of the transfection success<ref>PMID:3072883</ref>. '''NanoLuc luciferase''' is a small mutated luciferase from shrimp with enhanced stability and a significant increase in luminescence <ref>PMID:27045664</ref>. | |||
== 3D Structures of Luciferase == | |||
[[Luciferase 3D structures]] | |||
</StructureSection> | |||
==Additional information== | |||
[[Image:2d1s jena3dviewer image.jpg|left|220px]] | [[Image:2d1s jena3dviewer image.jpg|left|220px]] | ||
= | <scene name='Luciferase/2d1s/1'>Luciferase</scene> ([[Luciola cruciata luciferase|2d1s]]) {{Link Toggle FancyCartoonHighQualityView}} | ||
Several organisms are bioluminescent, meaning that they produce light. Luciferase is one of the enzymes that catalyzes light emitting reactions in organisms. | Several organisms are bioluminescent, meaning that they produce light. Luciferase is one of the enzymes that catalyzes light emitting reactions in organisms. | ||
{{Clear}} | |||
* For additional information, see: [[Colored & Bioluminescent Proteins]] | * For additional information, see: [[Colored & Bioluminescent Proteins]] | ||
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==Additional Literature== | ==Additional Literature== | ||
<ref group="xtra">PMID: 18949818</ref><ref group="xtra">PMID: 19859663</ref><references group="xtra"/> | <ref group="xtra">PMID: 18949818</ref><ref group="xtra">PMID: 19859663</ref><references group="xtra"/> | ||
==External Resources== | ==External Resources== | ||
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[[Category: Photoprotein]] | [[Category: Photoprotein]] | ||
[[Category: Alpha/beta-hydrolase]] | [[Category: Alpha/beta-hydrolase]] | ||
[[Category:Topic Page]] | |||
Latest revision as of 07:42, 11 July 2024
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Additional information
Luciferase (Luciola cruciata luciferase)
Several organisms are bioluminescent, meaning that they produce light. Luciferase is one of the enzymes that catalyzes light emitting reactions in organisms.
- For additional information, see: Luciferase FMN complex- Vibrio harveyi
References
Additional Literature
- Marques SM, Esteves da Silva JC. Firefly bioluminescence: a mechanistic approach of luciferase catalyzed reactions. IUBMB Life. 2009 Jan;61(1):6-17. PMID:18949818 doi:10.1002/iub.134
- Inouye S. Firefly luciferase: an adenylate-forming enzyme for multicatalytic functions. Cell Mol Life Sci. 2010 Feb;67(3):387-404. Epub 2009 Oct 27. PMID:19859663 doi:10.1007/s00018-009-0170-8
External Resources
- Yahoo news coverage of luciferase studies funded research for military applications
- Luciferase at Wikipedia
- Luciferase: June 2006 Molecule of the Month as part of the series of tutorials that are at the RCSB Protein Data Bank and written by David S. Goodsell
Proteopedia Page Contributors and Editors (what is this?)
Wayne Decatur, Alexander Berchansky, David Canner, Loïc Gazquez, Charlene Planchenault, Michal Harel
