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| [[Image:2jpz.gif|left|200px]]<br /><applet load="2jpz" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="2jpz" />
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| '''Structure of the hybrid-2 type intramolecular human telomeric G-quadruplex'''<br />
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| ==Overview== | | ==Human telomere DNA quadruplex structure in K+ solution hybrid-2 form== |
| Formation of the G-quadruplex in the human telomeric sequence can inhibit, the activity of telomerase, thus the intramolecular telomeric, G-quadruplexes have been considered as an attractive anticancer target., Information of intramolecular telomeric G-quadruplex structures formed, under physiological conditions is important for structure-based drug, design. Here, we report the first structure of the major intramolecular, G-quadruplex formed in a native, non-modified human telomeric sequence in, K(+) solution. This is a hybrid-type mixed, parallel/antiparallel-G-stranded G-quadruplex, one end of which is covered, by a novel T:A:T triple capping structure. This structure (Hybrid-2) and, the previously reported Hybrid-1 structure differ in their loop, arrangements, strand orientations and capping structures. The distinct, capping structures appear to be crucial for the favored formation of the, specific hybrid-type intramolecular telomeric G-quadruplexes, and may, provide specific binding sites for drug targeting. Our study also shows, that while the hybrid-type G-quadruplexes appear to be the major, conformations in K(+) solution, human telomeric sequences are always in, equilibrium between Hybrid-1 and Hybrid-2 structures, which is largely, determined by the 3'-flanking sequence. Furthermore, both hybrid-type, G-quadruplexes suggest a straightforward means for multimer formation with, effective packing in the human telomeric sequence and provide important, implications for drug targeting of G-quadruplexes in human telomeres.
| | <StructureSection load='2jpz' size='340' side='right'caption='[[2jpz]]' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[2jpz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JPZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JPZ FirstGlance]. <br> |
| 2JPZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JPZ OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jpz OCA], [https://pdbe.org/2jpz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jpz RCSB], [https://www.ebi.ac.uk/pdbsum/2jpz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jpz ProSAT]</span></td></tr> |
| ==Reference==
| | </table> |
| Structure of the Hybrid-2 type intramolecular human telomeric G-quadruplex in K+ solution: insights into structure polymorphism of the human telomeric sequence., Dai J, Carver M, Punchihewa C, Jones RA, Yang D, Nucleic Acids Res. 2007;35(15):4927-40. Epub 2007 Jul 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17626043 17626043]
| | __TOC__ |
| [[Category: Protein complex]] | | </StructureSection> |
| [[Category: Carver, M.]] | | [[Category: Large Structures]] |
| [[Category: Dai, J.]] | | [[Category: Carver M]] |
| [[Category: Jones, R.]] | | [[Category: Dai J]] |
| [[Category: Punchihewa, C.]] | | [[Category: Jones R]] |
| [[Category: Yang, D.]] | | [[Category: Punchihewa C]] |
| [[Category: dna]]
| | [[Category: Yang D]] |
| [[Category: human telomeric sequence]]
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| [[Category: nmr]]
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| [[Category: quadruplex]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:50:53 2008''
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