3no7: Difference between revisions

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{{Seed}}
[[Image:3no7.jpg|left|200px]]


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==Crystal structure of the centromere-binding protein ParB from plasmid pCXC100==
The line below this paragraph, containing "STRUCTURE_3no7", creates the "Structure Box" on the page.
<StructureSection load='3no7' size='340' side='right'caption='[[3no7]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3no7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leifsonia_xyli_subsp._cynodontis Leifsonia xyli subsp. cynodontis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NO7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NO7 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
{{STRUCTURE_3no7|  PDB=3no7  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3no7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3no7 OCA], [https://pdbe.org/3no7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3no7 RCSB], [https://www.ebi.ac.uk/pdbsum/3no7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3no7 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q6EEF9_LEIXC Q6EEF9_LEIXC]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Plasmid pCXC100 from the Gram-positive bacterium Leifsonia xyli subsp. cynodontis uses a type Ib partition system that includes a centromere region, a Walker-type ATPase ParA and a centromere-binding protein ParB for stable segregation. However, ParB shows no detectable sequence homology to any DNA-binding motif. Here, we study the ParB centromere interaction by structural and biochemical approaches. The crystal structure of the C-terminal DNA-binding domain of ParB at 1.4 A resolution reveals a dimeric ribbon-helix-helix (RHH) motif, supporting the prevalence of RHH motif in centromere binding. Using hydroxyl radical footprinting and quantitative binding assays, we show that the centromere core comprises nine uninterrupted 9-nt direct repeats that can be successively bound by ParB dimers in a cooperative manner. However, the interaction of ParB with a single subsite requires 18 base pairs covering one immediate repeat as well as two halves of flanking repeats. Through mutagenesis, sequence specificity was determined for each position of an 18-bp subsite. These data suggest an unique centromere recognition mechanism by which the repeat sequence is jointly specified by adjacent ParB dimers bound to an overlapped region.


===Crystal structure of the centromere-binding protein ParB from plasmid pCXC100===
Crystal structure and centromere binding of the plasmid segregation protein ParB from pCXC100.,Huang L, Yin P, Zhu X, Zhang Y, Ye K Nucleic Acids Res. 2010 Dec 1. PMID:21123191<ref>PMID:21123191</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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(as it appears on PubMed at http://www.pubmed.gov), where 21123191 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_21123191}}
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</StructureSection>
==About this Structure==
[[Category: Large Structures]]
3NO7 is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Leifsonia_xyli_subsp._cynodontis Leifsonia xyli subsp. cynodontis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NO7 OCA].
 
==Reference==
<ref group="xtra">PMID:21123191</ref><references group="xtra"/>
[[Category: Leifsonia xyli subsp. cynodontis]]
[[Category: Leifsonia xyli subsp. cynodontis]]
[[Category: Ye, K.]]
[[Category: Ye K]]
[[Category: Dna binding protein]]
[[Category: Ribbon-helix-helix]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 22 09:54:33 2010''
Retrieved from "https://proteopedia.org/3no7"