1u7g: Difference between revisions

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[[Image:1u7g.png|left|200px]]


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==Crystal Structure of Ammonia Channel AmtB from E. Coli==
The line below this paragraph, containing "STRUCTURE_1u7g", creates the "Structure Box" on the page.
<StructureSection load='1u7g' size='340' side='right'caption='[[1u7g]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1u7g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U7G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U7G FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NH3:AMMONIA'>NH3</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene></td></tr>
{{STRUCTURE_1u7g|  PDB=1u7g  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u7g OCA], [https://pdbe.org/1u7g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u7g RCSB], [https://www.ebi.ac.uk/pdbsum/1u7g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u7g ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AMTB_ECOLI AMTB_ECOLI] Involved in the uptake of ammonia.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u7/1u7g_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u7g ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The first structure of an ammonia channel from the Amt/MEP/Rh protein superfamily, determined to 1.35 angstrom resolution, shows it to be a channel that spans the membrane 11 times. Two structurally similar halves span the membrane with opposite polarity. Structures with and without ammonia or methyl ammonia show a vestibule that recruits NH4+/NH3, a binding site for NH4+, and a 20 angstrom-long hydrophobic channel that lowers the NH4+ pKa to below 6 and conducts NH3. Favorable interactions for NH3 are seen within the channel and use conserved histidines. Reconstitution of AmtB into vesicles shows that AmtB conducts uncharged NH3.


===Crystal Structure of Ammonia Channel AmtB from E. Coli===
Mechanism of ammonia transport by Amt/MEP/Rh: structure of AmtB at 1.35 A.,Khademi S, O'Connell J 3rd, Remis J, Robles-Colmenares Y, Miercke LJ, Stroud RM Science. 2004 Sep 10;305(5690):1587-94. PMID:15361618<ref>PMID:15361618</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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(as it appears on PubMed at http://www.pubmed.gov), where 15361618 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15361618}}
 
==About this Structure==
[[1u7g]] is a 1 chain structure of [[Ion channels (Part II)]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U7G OCA].


==See Also==
==See Also==
*[[Ion channels (Part II)]]
*[[Ion channels 3D structures|Ion channels 3D structures]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:15361618</ref><references group="xtra"/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: III, J O.Connell.]]
[[Category: Large Structures]]
[[Category: Khademi, S.]]
[[Category: Khademi S]]
[[Category: Miercke, L J.W.]]
[[Category: Miercke LJW]]
[[Category: Remis, J.]]
[[Category: O'Connell III J]]
[[Category: Robles-Colmenares, Y.]]
[[Category: Remis J]]
[[Category: Stroud, R M.]]
[[Category: Robles-Colmenares Y]]
[[Category: Ammonia channnel]]
[[Category: Stroud RM]]
[[Category: Membrane protein]]
[[Category: Rh protein]]
[[Category: Right handed helical bundle]]
[[Category: Transmembrane helice]]
[[Category: Transport protein]]
Retrieved from "https://proteopedia.org/1u7g"