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New page: left|200px<br /><applet load="2p6w" size="350" color="white" frame="true" align="right" spinBox="true" caption="2p6w, resolution 1.600Å" /> '''Crystal structure o...
 
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[[Image:2p6w.jpg|left|200px]]<br /><applet load="2p6w" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2p6w, resolution 1.600&Aring;" />
'''Crystal structure of a glycosyltransferase involved in the glycosylation of the major capsid of PBCV-1'''<br />


==Overview==
==Crystal structure of a glycosyltransferase involved in the glycosylation of the major capsid of PBCV-1==
Paramecium bursaria chlorella virus-1 encodes at least five putative, glycosyltransferases that are probably involved in the synthesis of the, glycan components of the viral major capsid protein. The 1.6 A crystal, structure of one of these glycosyltransferases (A64R) has a mixed, alpha/beta fold containing a central, six-stranded beta sheet flanked by, alpha helices. Crystal structures of A64R, complexed with UDP, CMP, or, GDP, established that only UDP bound to A64R in the presence of Mn(2+), consistent with its high structural similarity to glycosyltransferases, which utilize UDP as the sugar carrier. The structure of the complex of, A64R, UDP-glucose, and Mn(2+) showed that the largest conformational, change occurred when hydrogen bonds were formed with the ligands. Unlike, UDP-glucose, UDP-galactose and UDP-GlcNAc did not bind to A64R, suggesting, a selective binding of UDP-glucose. Thus, UDP-glucose is most likely the, sugar donor for A64R, consistent with glucose occurring in the virus major, capsid protein glycans.
<StructureSection load='2p6w' size='340' side='right'caption='[[2p6w]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2p6w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Paramecium_bursaria_Chlorella_virus_1 Paramecium bursaria Chlorella virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P6W FirstGlance]. <br>
2P6W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1] with <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=FLC:'>FLC</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6W OCA].
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p6w OCA], [https://pdbe.org/2p6w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p6w RCSB], [https://www.ebi.ac.uk/pdbsum/2p6w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p6w ProSAT]</span></td></tr>
Structure and function of a chlorella virus-encoded glycosyltransferase., Zhang Y, Xiang Y, Van Etten JL, Rossmann MG, Structure. 2007 Sep;15(9):1031-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17850743 17850743]
</table>
[[Category: Paramecium bursaria chlorella virus 1]]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/Q89399_PBCV1 Q89399_PBCV1]  
[[Category: Etten, J.L.Van.]]
__TOC__
[[Category: Rossmann, M.G.]]
</StructureSection>
[[Category: Ye, X.]]
[[Category: Large Structures]]
[[Category: Zhang, Y.]]
[[Category: Paramecium bursaria Chlorella virus 1]]
[[Category: FLC]]
[[Category: Rossmann MG]]
[[Category: MN]]
[[Category: Van Etten JL]]
[[Category: crystal structure]]
[[Category: Ye X]]
[[Category: glycosyltransferase]]
[[Category: Zhang Y]]
[[Category: pbcv]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:49:59 2008''

Latest revision as of 09:08, 21 February 2024

Crystal structure of a glycosyltransferase involved in the glycosylation of the major capsid of PBCV-1

2p6w, resolution 1.60Å

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