2pwo: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
New page: left|200px<br /><applet load="2pwo" size="350" color="white" frame="true" align="right" spinBox="true" caption="2pwo, resolution 1.450Å" /> '''Crystal Structure o...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(12 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:2pwo.jpg|left|200px]]<br /><applet load="2pwo" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2pwo, resolution 1.450&Aring;" />
'''Crystal Structure of HIV-1 CA146 A92E Psuedo Cell'''<br />


==Overview==
==Crystal Structure of HIV-1 CA146 A92E Psuedo Cell==
The CA domain of the human immunodeficiency virus type 1 (HIV-1) Gag, polyprotein plays critical roles in both the early and late phases of, viral replication and is therefore an attractive antiviral target., Compounds with antiviral activity were recently identified that bind to, the N-terminal domain of CA (CA(N)) and inhibit capsid assembly during, viral maturation. We have determined the structure of the complex between, CA(N) and the antiviral assembly inhibitor, N-(3-chloro-4-methylphenyl)-N'-{2-[({5-[(dimethylamino)-methyl]-2-furyl}-m, ethyl)-sulfanyl]ethyl}-urea) (CAP-1) using a combination of NMR, spectroscopy and X-ray crystallography. The protein undergoes a remarkable, conformational change upon CAP-1 binding, in which Phe32 is displaced from, its buried position in the protein core to open a deep hydrophobic cavity, that serves as the ligand binding site. The aromatic ring of CAP-1 inserts, into the cavity, with the urea NH groups forming hydrogen bonds with the, backbone oxygen of Val59 and the dimethylamonium group interacting with, the side-chains of Glu28 and Glu29. Elements that could be exploited to, improve binding affinity are apparent in the structure. The displacement, of Phe32 by CAP-1 appears to be facilitated by a strained main-chain, conformation, which suggests a potential role for a Phe32 conformational, switch during normal capsid assembly.
<StructureSection load='2pwo' size='340' side='right'caption='[[2pwo]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2pwo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PWO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PWO FirstGlance]. <br>
2PWO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1] with <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PWO OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pwo OCA], [https://pdbe.org/2pwo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pwo RCSB], [https://www.ebi.ac.uk/pdbsum/2pwo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pwo ProSAT]</span></td></tr>
Structure of the Antiviral Assembly Inhibitor CAP-1 Complex with the HIV-1 CA Protein., Kelly BN, Kyere S, Kinde I, Tang C, Howard BR, Robinson H, Sundquist WI, Summers MF, Hill CP, J Mol Biol. 2007 Aug 15;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17826792 17826792]
</table>
== Function ==
[https://www.uniprot.org/uniprot/A9PKC6_9HIV1 A9PKC6_9HIV1]
__TOC__
</StructureSection>
[[Category: Human immunodeficiency virus 1]]
[[Category: Human immunodeficiency virus 1]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Kelly, B.N.]]
[[Category: Kelly BN]]
[[Category: CL]]
[[Category: anti-viral]]
[[Category: hiv-1]]
[[Category: viral capsid]]
[[Category: viral protein]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:50:32 2008''

Latest revision as of 09:12, 21 February 2024

Crystal Structure of HIV-1 CA146 A92E Psuedo Cell

2pwo, resolution 1.45Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=2pwo&oldid=4062569"