3q20: Difference between revisions

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New page: '''Unreleased structure''' The entry 3q20 is ON HOLD Authors: Tarnawski, M., Krzywda, S., Szczepaniak, A., Jaskolski, M. Description: Crystal structure of RbcX C103A mutant from Thermo...
 
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'''Unreleased structure'''


The entry 3q20 is ON HOLD
==Crystal structure of RbcX C103A mutant from Thermosynechococcus elongatus==
<StructureSection load='3q20' size='340' side='right'caption='[[3q20]], [[Resolution|resolution]] 1.71&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3q20]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermosynechococcus_vestitus_BP-1 Thermosynechococcus vestitus BP-1]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3ka1 3ka1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q20 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q20 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.71&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q20 OCA], [https://pdbe.org/3q20 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q20 RCSB], [https://www.ebi.ac.uk/pdbsum/3q20 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q20 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RBCX_THEVB RBCX_THEVB] An RbcL-specific chaperone. Required for assembly of the RbcL8 core (PubMed:19081849) (Probable). The central cleft of the RbcX homodimer (RbcX2) binds the C-terminus of a RbcL monomer, stabilizing the C-terminus and probably preventing its reassociation with chaperonin GroEL-ES. At the same time the peripheral region of RbcX2 binds a second RbcL monomer, bridging the RbcL homodimers in the correct orientation. The RbcX2(2)-bound RbcL dimers then assemble into the RbcL8 core (RbcL8-(RbcX2)8). RbcS binding triggers the release of RbcX2 (By similarity).[UniProtKB:Q44212]<ref>PMID:19081849</ref> <ref>PMID:21821880</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of TeRbcX, a RuBisCO assembly chaperone from the cyanobacterium Thermosynechococcus elongatus, a thermophilic organism, has been determined at 1.7 A resolution. TeRbcX has an unusual cysteine residue at position 103 that is not found in RbcX proteins from mesophilic organisms. Unlike wild-type TeRbcX, a mutant protein with Cys103 replaced by Ala (TeRbcX-C103A) could be readily crystallized. The structure revealed that the overall fold of the TeRbcX homodimer is similar to those of previously crystallized RbcX proteins. Normal-mode analysis suggested that TeRbcX might adopt an open or closed conformation through a hinge movement pivoted on a kink in two long alpha4 helices. This type of conformational transition is presumably connected to RbcL (the large RuBisCO subunit) binding during the chaperone function of the RuBisCO assembly.


Authors: Tarnawski, M., Krzywda, S., Szczepaniak, A., Jaskolski, M.
Structure of the RuBisCO chaperone RbcX from the thermophilic cyanobacterium Thermosynechococcus elongatus.,Tarnawski M, Krzywda S, Bialek W, Jaskolski M, Szczepaniak A Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Aug 1;67(Pt, 8):851-7. Epub 2011 Jul 13. PMID:21821880<ref>PMID:21821880</ref>


Description: Crystal structure of RbcX C103A mutant from Thermosynechococcus elongatus
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3q20" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermosynechococcus vestitus BP-1]]
[[Category: Jaskolski M]]
[[Category: Krzywda S]]
[[Category: Szczepaniak A]]
[[Category: Tarnawski M]]
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