3q2r: Difference between revisions
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New page: '''Unreleased structure''' The entry 3q2r is ON HOLD Authors: Asojo, O.A. Description: crystal structure of sGLIPR1 soaked with zinc chloride |
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The | ==crystal structure of sGLIPR1 soaked with zinc chloride== | ||
<StructureSection load='3q2r' size='340' side='right'caption='[[3q2r]], [[Resolution|resolution]] 2.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3q2r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q2R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q2R FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q2r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q2r OCA], [https://pdbe.org/3q2r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q2r RCSB], [https://www.ebi.ac.uk/pdbsum/3q2r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q2r ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/GLIP1_HUMAN GLIP1_HUMAN] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Human glioma pathogenesis-related protein 1 (GLIPR1) is a membrane protein that is highly upregulated in brain cancers but is barely detectable in normal brain tissue. GLIPR1 is composed of a signal peptide that directs its secretion, a conserved cysteine-rich CAP (cysteine-rich secretory proteins, antigen 5 and pathogenesis-related 1 proteins) domain and a transmembrane domain. GLIPR1 is currently being investigated as a candidate for prostate cancer gene therapy and for glioblastoma targeted therapy. Crystal structures of a truncated soluble domain of the human GLIPR1 protein (sGLIPR1) solved by molecular replacement using a truncated polyalanine search model of the CAP domain of stecrisp, a snake-venom cysteine-rich secretory protein (CRISP), are presented. The correct molecular-replacement solution could only be obtained by removing all loops from the search model. The native structure was refined to 1.85 A resolution and that of a Zn(2+) complex was refined to 2.2 A resolution. The latter structure revealed that the putative binding cavity coordinates Zn(2+) similarly to snake-venom CRISPs, which are involved in Zn(2+)-dependent mechanisms of inflammatory modulation. Both sGLIPR1 structures have extensive flexible loop/turn regions and unique charge distributions that were not observed in any of the previously reported CAP protein structures. A model is also proposed for the structure of full-length membrane-bound GLIPR1. | |||
Structural studies of human glioma pathogenesis-related protein 1.,Asojo OA, Koski RA, Bonafe N Acta Crystallogr D Biol Crystallogr. 2011 Oct;67(Pt 10):847-55. Epub 2011, Sep 8. PMID:21931216<ref>PMID:21931216</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3q2r" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Asojo OA]] | |||
Latest revision as of 09:35, 30 October 2024
crystal structure of sGLIPR1 soaked with zinc chloride
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