3o62: Difference between revisions

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[[Image:3o62.jpg|left|200px]]


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==Nucleosome core particle modified with a cisplatin 1,3-cis-{Pt(NH3)2}2+-d(GpTpG) intrastrand cross-link==
The line below this paragraph, containing "STRUCTURE_3o62", creates the "Structure Box" on the page.
<StructureSection load='3o62' size='340' side='right'caption='[[3o62]], [[Resolution|resolution]] 3.22&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3o62]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O62 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O62 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.216&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CPT:CISPLATIN'>CPT</scene></td></tr>
{{STRUCTURE_3o62|  PDB=3o62  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o62 OCA], [https://pdbe.org/3o62 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o62 RCSB], [https://www.ebi.ac.uk/pdbsum/3o62 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o62 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/H32_XENLA H32_XENLA] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The effects of cisplatin binding to DNA were explored at the nucleosome level to incorporate key features of the eukaryotic nuclear environment. An X-ray crystal structure of a site-specifically platinated nucleosome carrying a 1,3-cis-{Pt(NH)}(2)+-d(GpTpG) intrastrand cross-link reveals the details of how this adduct dictates the rotational positioning of DNA in the nucleosome. Results from in vitro nucleosome mobility assays indicate that a single platinum adduct interferes with ATP-independent sliding of DNA around the octamer core. Data from in vitro transcription experiments suggest that RNA polymerases can successfully navigate along cisplatin-damaged DNA templates that contain nucleosomes, but stall when the transcription elongation complex physically contacts a platinum cross-link located on the template strand. These results provide information about the effects of cisplatin binding to nuclear DNA and enhance our understanding of the mechanism of transcription inhibition by platinum anticancer compounds.


===Nucleosome core particle modified with a cisplatin 1,3-cis-{Pt(NH3)2}2+-d(GpTpG) intrastrand cross-link===
Consequences of cisplatin binding on nucleosome structure and dynamics.,Todd RC, Lippard SJ Chem Biol. 2010 Dec 22;17(12):1334-43. PMID:21168769<ref>PMID:21168769</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3o62" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_21168769}}, adds the Publication Abstract to the page
*[[Histone 3D structures|Histone 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 21168769 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_21168769}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
[[3o62]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O62 OCA].
 
==Reference==
<ref group="xtra">PMID:21168769</ref><references group="xtra"/>
[[Category: Xenopus laevis]]
[[Category: Xenopus laevis]]
[[Category: Lippard, S J.]]
[[Category: Lippard SJ]]
[[Category: Todd, R C.]]
[[Category: Todd RC]]

Latest revision as of 09:56, 13 August 2026

Nucleosome core particle modified with a cisplatin 1,3-cis-{Pt(NH3)2}2+-d(GpTpG) intrastrand cross-link

3o62, resolution 3.22Å

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