2kjo: Difference between revisions

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[[Image:2kjo.png|left|200px]]


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==pH dependent structures of LAH4 in micellar environment: mode of acting==
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<StructureSection load='2kjo' size='340' side='right'caption='[[2kjo]]' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2kjo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KJO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KJO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kjo OCA], [https://pdbe.org/2kjo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kjo RCSB], [https://www.ebi.ac.uk/pdbsum/2kjo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kjo ProSAT]</span></td></tr>
{{STRUCTURE_2kjo|  PDB=2kjo  |  SCENE=  }}
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== Publication Abstract from PubMed ==
The LAH4 family of histidine-rich peptides exhibits potent antimicrobial and DNA transfection activities, both of which require interactions with cellular membranes. The bilayer association of the peptides has been shown to be strongly pH-dependent, with in-planar alignments under acidic conditions and transmembrane orientations when the histidines are discharged. Therefore, we investigated the pH- and temperature-dependent conformations of LAH4 in DPC micellar solutions and in a TFE/PBS solvent mixture. In the presence of detergent and at pH 4.1, LAH4 adopts helical conformations between residues 9 and 24 concomitantly with a high hydrophobic moment. At pH 6.1, a helix-loop-helix structure forms with a hinge encompassing residues His(1)-Ala(1)(3). The data suggest that the high density of histidine residues and the resulting electrostatic repulsion lead to both a decrease in the pK values of the histidines and a less stable alpha-helical conformation of this region. The hinged structure at pH 6.1 facilitates membrane anchoring and insertion. At pH 7.8, the histidines are uncharged and an extended helical conformation including residues 4-21 is again obtained. LAH4 thus exhibits a high degree of conformational plasticity. The structures provide a stroboscopic view of the conformational changes that occur during membrane insertion, and are discussed in the context of antimicrobial activity and DNA transfection.


===pH dependent structures of LAH4 in micellar environment: mode of acting===
NMR structures of the histidine-rich peptide LAH4 in micellar environments: membrane insertion, pH-dependent mode of antimicrobial action, and DNA transfection.,Georgescu J, Munhoz VH, Bechinger B Biophys J. 2010 Oct 20;99(8):2507-15. PMID:20959091<ref>PMID:20959091</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
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<references/>
{{ABSTRACT_PUBMED_20959091}}
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</StructureSection>
==About this Structure==
[[Category: Large Structures]]
[[2kjo]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KJO OCA].
[[Category: Synthetic construct]]
 
[[Category: Bechinger B]]
==Reference==
[[Category: Georgescu J]]
<ref group="xtra">PMID:20959091</ref><references group="xtra"/>
[[Category: Bechinger, B.]]
[[Category: Georgescu, J.]]
Retrieved from "https://proteopedia.org/2kjo"