2r0c: Difference between revisions

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New page: left|200px<br /><applet load="2r0c" size="350" color="white" frame="true" align="right" spinBox="true" caption="2r0c, resolution 1.80Å" /> '''Structure of the sub...
 
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[[Image:2r0c.gif|left|200px]]<br /><applet load="2r0c" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2r0c, resolution 1.80&Aring;" />
'''Structure of the substrate-free form of the rebeccamycin biosynthetic enzyme REBC'''<br />


==Overview==
==Structure of the substrate-free form of the rebeccamycin biosynthetic enzyme REBC==
The biosynthesis of rebeccamycin, an antitumor compound, involves the, remarkable eight-electron oxidation of chlorinated chromopyrrolic acid., Although one rebeccamycin biosynthetic enzyme is capable of generating low, levels of the eight-electron oxidation product on its own, a second, protein, RebC, is required to accelerate product formation and eliminate, side reactions. However, the mode of action of RebC was largely unknown., Using crystallography, we have determined a likely function for RebC as a, flavin hydroxylase, captured two snapshots of its dynamic catalytic cycle, and trapped a reactive molecule, a putative substrate, in its binding, pocket. These studies strongly suggest that the role of RebC is to, sequester a reactive intermediate produced by its partner protein and to, react with it enzymatically, preventing its conversion to a suite of, degradation products that includes, at low levels, the desired product.
<StructureSection load='2r0c' size='340' side='right'caption='[[2r0c]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2r0c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lentzea_aerocolonigenes Lentzea aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R0C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R0C FirstGlance]. <br>
2R0C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lechevalieria_aerocolonigenes Lechevalieria aerocolonigenes] with <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=FAD:'>FAD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R0C OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r0c OCA], [https://pdbe.org/2r0c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r0c RCSB], [https://www.ebi.ac.uk/pdbsum/2r0c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r0c ProSAT]</span></td></tr>
Crystallographic trapping in the rebeccamycin biosynthetic enzyme RebC., Ryan KS, Howard-Jones AR, Hamill MJ, Elliott SJ, Walsh CT, Drennan CL, Proc Natl Acad Sci U S A. 2007 Sep 14;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17873060 17873060]
</table>
[[Category: Lechevalieria aerocolonigenes]]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/Q8KI25_LENAE Q8KI25_LENAE]  
[[Category: Drennan, C.L.]]
== Evolutionary Conservation ==
[[Category: Ryan, K.S.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: CL]]
Check<jmol>
[[Category: FAD]]
  <jmolCheckbox>
[[Category: flavin adenine dinucleotide]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r0/2r0c_consurf.spt"</scriptWhenChecked>
[[Category: monooxygenase]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: oxidoreductase]]
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:30:04 2008''
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r0c ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Lentzea aerocolonigenes]]
[[Category: Drennan CL]]
[[Category: Ryan KS]]

Latest revision as of 09:19, 21 February 2024

Structure of the substrate-free form of the rebeccamycin biosynthetic enzyme REBC

2r0c, resolution 1.80Å

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