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New page: left|200px<br /><applet load="2ckd" size="350" color="white" frame="true" align="right" spinBox="true" caption="2ckd, resolution 2.80Å" /> '''CRYSTAL STRUCTURE OF...
 
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[[Image:2ckd.jpg|left|200px]]<br /><applet load="2ckd" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2ckd, resolution 2.80&Aring;" />
'''CRYSTAL STRUCTURE OF ML2640 FROM MYCOBACTERIUM LEPRAE'''<br />


==Overview==
==Crystal structure of ML2640 from Mycobacterium leprae==
Mycobacterium leprae protein ML2640c belongs to a large family of, conserved hypothetical proteins predominantly found in mycobacteria, some, of them predicted as putative S-adenosylmethionine (AdoMet)-dependent, methyltransferases (MTase). As part of a Structural Genomics initiative on, conserved hypothetical proteins in pathogenic mycobacteria, we have, determined the structure of ML2640c in two distinct crystal forms. As, expected, ML2640c has a typical MTase core domain and binds the methyl, donor substrate AdoMet in a manner consistent with other known members of, this structural family. The putative acceptor substrate-binding site of, ML2640c is a large internal cavity, mostly lined by aromatic and aliphatic, side-chain residues, suggesting that a lipid-like molecule might be, targeted for catalysis. A flap segment (residues 222-256), which isolates, the binding site from the bulk solvent and is highly mobile in the crystal, structures, could serve as a gateway to allow substrate entry and product, release. The multiple sequence alignment of ML2640c-like proteins revealed, that the central alpha/beta core and the AdoMet-binding site are very well, conserved within the family. However, the amino acid positions defining, the binding site for the acceptor substrate display a higher variability, suggestive of distinct acceptor substrate specificities. The ML2640c, crystal structures offer the first structural glimpses at this important, family of mycobacterial proteins and lend strong support to their, functional assignment as AdoMet-dependent methyltransferases.
<StructureSection load='2ckd' size='340' side='right'caption='[[2ckd]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2ckd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_leprae Mycobacterium leprae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CKD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CKD FirstGlance]. <br>
2CKD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_leprae Mycobacterium leprae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CKD OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ckd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ckd OCA], [https://pdbe.org/2ckd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ckd RCSB], [https://www.ebi.ac.uk/pdbsum/2ckd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ckd ProSAT]</span></td></tr>
==Reference==
</table>
The crystal structure of M. leprae ML2640c defines a large family of putative S-adenosylmethionine-dependent methyltransferases in mycobacteria., Grana M, Haouz A, Buschiazzo A, Miras I, Wehenkel A, Bondet V, Shepard W, Schaeffer F, Cole ST, Alzari PM, Protein Sci. 2007 Sep;16(9):1896-904. Epub 2007 Jul 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17660248 17660248]
== Function ==
[https://www.uniprot.org/uniprot/Y2640_MYCLE Y2640_MYCLE] Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity.<ref>PMID:17660248</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ck/2ckd_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ckd ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mycobacterium leprae]]
[[Category: Mycobacterium leprae]]
[[Category: Single protein]]
[[Category: Alzari PM]]
[[Category: Alzari, P.M.]]
[[Category: Buschiazzo A]]
[[Category: Buschiazzo, A.]]
[[Category: Grana M]]
[[Category: Grana, M.]]
[[Category: Haouz A]]
[[Category: Haouz, A.]]
[[Category: Wehenkel A]]
[[Category: Wehenkel, A.]]
[[Category: hypothetical protein]]
[[Category: transferase]]
 
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