2z7a: Difference between revisions

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New page: left|200px<br /><applet load="2z7a" size="350" color="white" frame="true" align="right" spinBox="true" caption="2z7a, resolution 2.10Å" /> '''X-ray crystal struct...
 
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[[Image:2z7a.gif|left|200px]]<br /><applet load="2z7a" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2z7a, resolution 2.10&Aring;" />
'''X-ray crystal structure of RV0760c from Mycobacterium tuberculosis at 2.10 Angstrom resolution'''<br />


==About this Structure==
==X-ray crystal structure of RV0760c from Mycobacterium tuberculosis at 2.10 Angstrom resolution==
2Z7A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv] with <scene name='pdbligand=ACT:'>ACT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z7A OCA].
<StructureSection load='2z7a' size='340' side='right'caption='[[2z7a]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
[[Category: Mycobacterium tuberculosis h37rv]]
== Structural highlights ==
[[Category: Single protein]]
<table><tr><td colspan='2'>[[2z7a]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z7A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z7A FirstGlance]. <br>
[[Category: Cherney, M.M.]]
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
[[Category: Garen, C.R.]]
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
[[Category: James, M.N.G.]]
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z7a OCA], [https://pdbe.org/2z7a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z7a RCSB], [https://www.ebi.ac.uk/pdbsum/2z7a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z7a ProSAT], [https://www.topsan.org/Proteins/TBSGC/2z7a TOPSAN]</span></td></tr>
[[Category: TBSGC, TB.Structural.Genomics.Consortium.]]
</table>
[[Category: ACT]]
== Function ==
[[Category: alpha+beta conical barrel]]
[https://www.uniprot.org/uniprot/P71817_MYCTO P71817_MYCTO]  
[[Category: isomerase]]
== Evolutionary Conservation ==
[[Category: structural genomics]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: tb structural genomics consortium]]
Check<jmol>
[[Category: tbsgc]]
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z7/2z7a_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z7a ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We have determined the X-ray crystal structure of the Mycobacterium tuberculosis (Mtb) gene product encoded by the open reading frame Rv0760c at 1.50 A resolution by single-wavelength anomalous dispersion (SAD) phasing of diffraction data from crystals of the selenomethionine-substituted protein. Refinement against diffraction data from the native protein resulted in R(work)=19.5% and R(free)=21.4%. The X-ray crystal structure shows that the homodimeric Rv0760c polypeptide has an alpha + beta conical barrel fold placing it among many structural neighbors of the nuclear transport factor 2 family (NTF2). This family is highly conserved in terms of structure; however the substrates and individual protein functions are diverse. The structures of native Rv0760c in several different crystal forms and Rv0760c bound to 17beta-estradiol 17-hemisuccinate (EH) have also been solved and analyzed.


''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:42:02 2008''
Crystal structure of Mycobacterium tuberculosis Rv0760c at 1.50 A resolution, a structural homolog of Delta(5)-3-ketosteroid isomerase.,Cherney MM, Garen CR, James MN Biochim Biophys Acta. 2008 Nov;1784(11):1625-32. Epub 2008 Jun 6. PMID:18589008<ref>PMID:18589008</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2z7a" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis H37Rv]]
[[Category: Cherney MM]]
[[Category: Garen CR]]
[[Category: James MNG]]
Retrieved from "https://proteopedia.org/2z7a"