3qjo: Difference between revisions
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New page: '''Unreleased structure''' The entry 3qjo is ON HOLD Authors: Jaenicke, E., Buchler, K., Decker, H. , Markl, J., Schroder, G.F. Description: Refined Structure of the functional unit (K... |
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The | ==Refined Structure of the functional unit (KLH1-H) of keyhole limpet hemocyanin== | ||
<StructureSection load='3qjo' size='340' side='right'caption='[[3qjo]], [[Resolution|resolution]] 4.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3qjo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Megathura_crenulata Megathura crenulata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QJO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QJO FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qjo OCA], [https://pdbe.org/3qjo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qjo RCSB], [https://www.ebi.ac.uk/pdbsum/3qjo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qjo ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/HCY1_MEGCR HCY1_MEGCR] Hemocyanins are copper-containing oxygen carriers occurring freely dissolved in the hemolymph of many mollusks and arthropods.<ref>PMID:8829804</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Hemocyanins are multimeric oxygen-transport proteins in the hemolymph of many arthropods and mollusks. The overall molecular architecture of arthropod and molluscan hemocyanin is very different, although they possess a similar binuclear type 3 copper center to bind oxygen in a side-on conformation. Gastropod hemocyanin is a 35 nm cylindrical didecamer (2 x 10-mer) based on a 400 kDa subunit. The latter is subdivided into eight paralogous "functional units" (FU-a to FU-h), each with an active site. FU-a to FU-f contribute to the cylinder wall, whereas FU-g and FU-h form the internal collar complex. Atomic structures of FU-e and FU-g, and a 9 A cryoEM structure of the 8 MDa didecamer are available. Recently, the structure of keyhole limpet hemocyanin FU-h (KLH1-h) was presented as a C(alpha) -trace at 4 A resolution. Unlike the other seven FU types, FU-h contains an additional C-terminal domain with a cupredoxin-like fold. Because of the resolution limit of 4 A, in some loops, the course of the protein backbone could not be established with high certainty yet. Here, we present a refined atomic structure of FU-h (KLH1-h) obtained from low-resolution refinement, which unambiguously establishes the course of the polypeptide backbone and reveals the disulfide bridges as well as the orientation of bulky amino acids. | |||
The refined structure of functional unit h of keyhole limpet hemocyanin (KLH1-h) reveals disulfide bridges.,Jaenicke E, Buchler K, Decker H, Markl J, Schroder GF IUBMB Life. 2011 Mar;63(3):183-7. doi: 10.1002/iub.435. PMID:21445849<ref>PMID:21445849</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3qjo" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Megathura crenulata]] | |||
[[Category: Buchler K]] | |||
[[Category: Decker H]] | |||
[[Category: Jaenicke E]] | |||
[[Category: Markl J]] | |||
[[Category: Schroder GF]] | |||
