3p3o: Difference between revisions
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< | ==Crystal Structure of the Cytochrome P450 Monooxygenase AurH (ntermII) from Streptomyces Thioluteus== | ||
<StructureSection load='3p3o' size='340' side='right'caption='[[3p3o]], [[Resolution|resolution]] 1.54Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3p3o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_thioluteus Streptomyces thioluteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P3O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P3O FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.54Å</td></tr> | |||
- | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p3o OCA], [https://pdbe.org/3p3o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p3o RCSB], [https://www.ebi.ac.uk/pdbsum/3p3o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p3o ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/AURH_STRTU AURH_STRTU] Bifunctional cytochrome P450 protein involved in the biosynthesis of the antibiotic aureothin, a nitroaryl polyketide metabolite with antifungal, cytotoxic and insecticidal activities (PubMed:15612710, PubMed:18855960). Catalyzes the hydroxylation of luteothin (also called deoxyaureothin), leading to the formation of the intermediate (7R)-7-hydroxydeoxyaureothin, followed by the formation of the aureothin tetrahydrofuran ring, the final step in the biosynthesis of aureothin (PubMed:15612710, PubMed:18855960, PubMed:21280577).<ref>PMID:15612710</ref> <ref>PMID:18855960</ref> <ref>PMID:21280577</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
AurH is a unique cytochrome P450 monooxygenase catalyzing the stepwise formation of a homochiral oxygen heterocycle, a key structural and pharmacophoric component of the antibiotic aureothin. The exceptional enzymatic reaction involves a tandem oxygenation process including a regio- and stereospecific hydroxylation, followed by heterocyclization. For the structural and biochemical basis of this unparalleled sequence, four crystal structures of AurH variants in different conformational states and in complex with the P450 inhibitor ancymidol were solved, which represent the first structures of the CYP151A group. Structural data in conjunction with computational docking, site-directed mutagenesis, and chemical analyses unveiled a switch function when recognizing the two substrates, deoxyaureothin and the hydroxylated intermediate, thus allowing the second oxygenation-heterocyclization step. Furthermore, we were able to modify the chemo- and regioselectivity of AurH, yielding mutants that catalyze the regioselective six-electron transfer of a nonactivated methyl group to a carboxylic acid via hydroxyl and aldehyde intermediates. | |||
Structural Fine-Tuning of a Multifunctional Cytochrome P450 Monooxygenase.,Zocher G, Richter ME, Mueller U, Hertweck C J Am Chem Soc. 2011 Jan 31. PMID:21280577<ref>PMID:21280577</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3p3o" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | [[Category: Large Structures]] | ||
[[ | |||
== | |||
< | |||
[[Category: Streptomyces thioluteus]] | [[Category: Streptomyces thioluteus]] | ||
[[Category: Hertweck | [[Category: Hertweck C]] | ||
[[Category: Mueller | [[Category: Mueller U]] | ||
[[Category: Richter | [[Category: Richter MEA]] | ||
[[Category: Zocher | [[Category: Zocher G]] | ||
Latest revision as of 09:59, 13 August 2026
Crystal Structure of the Cytochrome P450 Monooxygenase AurH (ntermII) from Streptomyces Thioluteus
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