3pzf: Difference between revisions

Latest revision as of 10:44, 21 February 2024

1.75A resolution structure of Serpin-2 from Anopheles gambiae

Structural highlights

3pzf is a 1 chain structure with sequence from Anopheles gambiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.75Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SP2_ANOGA Serine protease inhibitor that functions in the melanization-mediated immune response (PubMed:16113656, PubMed:20953892, PubMed:33520733, PubMed:33045027, PubMed:21465556, PubMed:25525260). By preventing the activation of phenoloxidases through the inhibiting of serine proteases CLIPB9 and CLIPB10, negatively regulates melanization in the hemolymph (PubMed:16113656, PubMed:20953892, PubMed:33520733, PubMed:33045027, PubMed:21465556, PubMed:25525260). By preventing melanization, has a detrimental role during P.berghei parasite mediated-infection and invasion of the mosquito midgut (PubMed:16113656).^[1] ^[2] ^[3] ^[4] ^[5] ^[6]

References

↑ Michel K, Budd A, Pinto S, Gibson TJ, Kafatos FC. Anopheles gambiae SRPN2 facilitates midgut invasion by the malaria parasite Plasmodium berghei. EMBO Rep. 2005 Sep;6(9):891-7. PMID:16113656 doi:10.1038/sj.embor.7400478
↑ An C, Budd A, Kanost MR, Michel K. Characterization of a regulatory unit that controls melanization and affects longevity of mosquitoes. Cell Mol Life Sci. 2011 Jun;68(11):1929-39. PMID:20953892 doi:10.1007/s00018-010-0543-z
↑ An C, Lovell S, Kanost MR, Battaile KP, Michel K. Crystal structure of native Anopheles gambiae serpin-2, a negative regulator of melanization in mosquitoes. Proteins. 2011 Feb 3. doi: 10.1002/prot.23002. PMID:21465556 doi:10.1002/prot.23002
↑ Zhang X, Meekins DA, An C, Zolkiewski M, Battaile KP, Kanost MR, Lovell S, Michel K. Structural and Inhibitory Effects of Hinge Loop Mutagenesis in Serpin-2 from the Malaria Vector Anopheles gambiae. J Biol Chem. 2015 Jan 30;290(5):2946-56. doi: 10.1074/jbc.M114.625665. Epub 2014 , Dec 17. PMID:25525260 doi:https://dx.doi.org/10.1074/jbc.M114.625665
↑ Sousa GL, Bishnoi R, Baxter RHG, Povelones M. The CLIP-domain serine protease CLIPC9 regulates melanization downstream of SPCLIP1, CLIPA8, and CLIPA28 in the malaria vector Anopheles gambiae. PLoS Pathog. 2020 Oct 12;16(10):e1008985. PMID:33045027 doi:10.1371/journal.ppat.1008985
↑ Zhang X, Li M, El Moussawi L, Saab S, Zhang S, Osta MA, Michel K. CLIPB10 is a Terminal Protease in the Regulatory Network That Controls Melanization in the African Malaria Mosquito Anopheles gambiae. Front Cell Infect Microbiol. 2021 Jan 15;10:585986. PMID:33520733 doi:10.3389/fcimb.2020.585986

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Michel K, Budd A, Pinto S, Gibson TJ, Kafatos FC. Anopheles gambiae SRPN2 facilitates midgut invasion by the malaria parasite Plasmodium berghei. EMBO Rep. 2005 Sep;6(9):891-7. PMID:16113656 doi:10.1038/sj.embor.7400478

[2] An C, Budd A, Kanost MR, Michel K. Characterization of a regulatory unit that controls melanization and affects longevity of mosquitoes. Cell Mol Life Sci. 2011 Jun;68(11):1929-39. PMID:20953892 doi:10.1007/s00018-010-0543-z

[3] An C, Lovell S, Kanost MR, Battaile KP, Michel K. Crystal structure of native Anopheles gambiae serpin-2, a negative regulator of melanization in mosquitoes. Proteins. 2011 Feb 3. doi: 10.1002/prot.23002. PMID:21465556 doi:10.1002/prot.23002

[4] Zhang X, Meekins DA, An C, Zolkiewski M, Battaile KP, Kanost MR, Lovell S, Michel K. Structural and Inhibitory Effects of Hinge Loop Mutagenesis in Serpin-2 from the Malaria Vector Anopheles gambiae. J Biol Chem. 2015 Jan 30;290(5):2946-56. doi: 10.1074/jbc.M114.625665. Epub 2014 , Dec 17. PMID:25525260 doi:https://dx.doi.org/10.1074/jbc.M114.625665

[5] Sousa GL, Bishnoi R, Baxter RHG, Povelones M. The CLIP-domain serine protease CLIPC9 regulates melanization downstream of SPCLIP1, CLIPA8, and CLIPA28 in the malaria vector Anopheles gambiae. PLoS Pathog. 2020 Oct 12;16(10):e1008985. PMID:33045027 doi:10.1371/journal.ppat.1008985

[6] Zhang X, Li M, El Moussawi L, Saab S, Zhang S, Osta MA, Michel K. CLIPB10 is a Terminal Protease in the Regulatory Network That Controls Melanization in the African Malaria Mosquito Anopheles gambiae. Front Cell Infect Microbiol. 2021 Jan 15;10:585986. PMID:33520733 doi:10.3389/fcimb.2020.585986

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@@ Line 1: / Line 1: @@
-[[Image:3pzf.jpg|left|200px]]
-<!--
+==1.75A resolution structure of Serpin-2 from Anopheles gambiae==
-The line below this paragraph, containing "STRUCTURE_3pzf", creates the "Structure Box" on the page.
+<StructureSection load='3pzf' size='340' side='right'caption='[[3pzf]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3pzf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Anopheles_gambiae Anopheles gambiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PZF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PZF FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pzf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pzf OCA], [https://pdbe.org/3pzf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pzf RCSB], [https://www.ebi.ac.uk/pdbsum/3pzf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pzf ProSAT]</span></td></tr>
-{{STRUCTURE_3pzf|  PDB=3pzf  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SP2_ANOGA SP2_ANOGA] Serine protease inhibitor that functions in the melanization-mediated immune response (PubMed:16113656, PubMed:20953892, PubMed:33520733, PubMed:33045027, PubMed:21465556, PubMed:25525260). By preventing the activation of phenoloxidases through the inhibiting of serine proteases CLIPB9 and CLIPB10, negatively regulates melanization in the hemolymph (PubMed:16113656, PubMed:20953892, PubMed:33520733, PubMed:33045027, PubMed:21465556, PubMed:25525260). By preventing melanization, has a detrimental role during P.berghei parasite mediated-infection and invasion of the mosquito midgut (PubMed:16113656).<ref>PMID:16113656</ref> <ref>PMID:20953892</ref> <ref>PMID:21465556</ref> <ref>PMID:25525260</ref> <ref>PMID:33045027</ref> <ref>PMID:33520733</ref>
-===1.75A resolution structure of Serpin-2 from Anopheles gambiae===
+==See Also==
+*[[Serpin 3D structures|Serpin 3D structures]]
+== References ==
-==About this Structure==
+<references/>
-[[3pzf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Anopheles_gambiae Anopheles gambiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PZF OCA].
+__TOC__
+</StructureSection>
 [[Category: Anopheles gambiae]]
-[[Category: An, C.]]
+[[Category: Large Structures]]
-[[Category: Battaile, K P.]]
+[[Category: An C]]
-[[Category: Lovell, S.]]
+[[Category: Battaile KP]]
-[[Category: Michel, K.]]
+[[Category: Lovell S]]
+[[Category: Michel K]]

3pzf: Difference between revisions

Latest revision as of 10:44, 21 February 2024

1.75A resolution structure of Serpin-2 from Anopheles gambiae

Structural highlights

Function

See Also

References

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