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[[Image:2fvy.jpg|left|200px]]<br /><applet load="2fvy" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2fvy, resolution 0.92&Aring;" />
'''High Resolution Glucose Bound Crystal Structure of GGBP'''<br />


==Overview==
==High Resolution Glucose Bound Crystal Structure of GGBP==
D-Glucose/D-Galactose-binding protein (GGBP) mediates chemotaxis toward, and active transport of glucose and galactose in a number of bacterial, species. GGBP, like other periplasmic binding proteins, can exist in open, (ligand-free) and closed (ligand-bound) states. We report a 0.92 angstroms, resolution structure of GGBP from Escherichia coli in the glucose-bound, state and the first structure of an open, unbound form of GGBP (at 1.55, angstroms resolution). These structures vary in the angle between the two, structural domains; the observed difference of 31 degrees arises from, torsion angle changes in a three-segment hinge. A comparison with the, closely related periplasmic receptors, ribose- and allose-binding, proteins, shows that the GGBP hinge residue positions that undergo the, largest conformational changes are different. Furthermore, the, high-quality data collected for the atomic resolution glucose-bound, structure allow for the refinement of specific hydrogen atom positions, the assignment of alternate side chain conformations, the first, description of CO(2) trapped after radiation-induced decarboxylation, and, insight into the role of the exo-anomeric effect in sugar binding., Together, these structures provide insight into how the hinge-bending, movement of GGBP facilitates ligand binding, transport, and signaling.
<StructureSection load='2fvy' size='340' side='right'caption='[[2fvy]], [[Resolution|resolution]] 0.92&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2fvy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FVY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FVY FirstGlance]. <br>
2FVY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=GLC:'>GLC</scene>, <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=ACT:'>ACT</scene>, <scene name='pdbligand=CO2:'>CO2</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FVY OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.92&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CO2:CARBON+DIOXIDE'>CO2</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fvy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fvy OCA], [https://pdbe.org/2fvy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fvy RCSB], [https://www.ebi.ac.uk/pdbsum/2fvy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fvy ProSAT]</span></td></tr>
Conformational changes of glucose/galactose-binding protein illuminated by open, unliganded, and ultra-high-resolution ligand-bound structures., Borrok MJ, Kiessling LL, Forest KT, Protein Sci. 2007 Jun;16(6):1032-41. Epub 2007 May 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17473016 17473016]
</table>
== Function ==
[https://www.uniprot.org/uniprot/MGLB_ECOLI MGLB_ECOLI] Part of the ABC transporter complex MglABC involved in galactose/methyl galactoside import (Probable). In addition, binds D-galactose and D-glucose and plays a role in the chemotaxis towards these two sugars by interacting with the Trg chemoreceptor (PubMed:3057628, PubMed:4927373). Chemotaxis requires MglB, but not MglA or MglC (PubMed:6294056).<ref>PMID:3057628</ref> <ref>PMID:4927373</ref> <ref>PMID:6294056</ref> <ref>PMID:1719366</ref> <ref>PMID:6294056</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fv/2fvy_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fvy ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Borrok, M.J.]]
[[Category: Borrok MJ]]
[[Category: Forest, K.T.]]
[[Category: Forest KT]]
[[Category: Kiessling, L.L.]]
[[Category: Kiessling LL]]
[[Category: ACT]]
[[Category: CA]]
[[Category: CO2]]
[[Category: GLC]]
[[Category: GOL]]
[[Category: chemotaxis]]
[[Category: hinge]]
[[Category: periplasmic binding protien]]
[[Category: transport]]
 
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Retrieved from "https://proteopedia.org/2fvy"