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New page: left|200px<br /><applet load="2ox5" size="350" color="white" frame="true" align="right" spinBox="true" caption="2ox5, resolution 1.980Å" /> '''The SoxYZ complex o...
 
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[[Image:2ox5.jpg|left|200px]]<br /><applet load="2ox5" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2ox5, resolution 1.980&Aring;" />
'''The SoxYZ complex of Paracoccus pantotrophus'''<br />


==Overview==
==The SoxYZ complex of Paracoccus pantotrophus==
The bacterial Sox (sulfur oxidizing) system allows the utilization of, inorganic sulfur compounds in energy metabolism. Central to this process, is the SoxYZ complex that carries the pathway intermediates on a cysteine, residue near the C terminus of SoxY. Crystal structures have been, determined for Paracoccus pantotrophus SoxYZ with the carrier cysteine in, the underivatized state, conjugated to the polysulfide mimic, beta-mercaptoethanol, and as the sulfonate adduct pathway intermediate., The carrier cysteine is located on a peptide swinging arm and is bracketed, on either side by diglycine dipeptides acting as molecular universal, joints. This structure provides a novel solution to the requirement that, the cysteine-bound intermediates be able to access and orient themselves, within the active sites of multiple partner enzymes. Adjacent to the, swinging arm there is a conserved, deep, apolar pocket into which the, beta-mercaptoethanol adduct extends. This pocket would be well suited to a, role in protecting labile pathway intermediates from adventitious, reactions.
<StructureSection load='2ox5' size='340' side='right'caption='[[2ox5]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2ox5]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Paracoccus_denitrificans Paracoccus denitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OX5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OX5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.98&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ox5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ox5 OCA], [https://pdbe.org/2ox5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ox5 RCSB], [https://www.ebi.ac.uk/pdbsum/2ox5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ox5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9LCU8_PARDE Q9LCU8_PARDE]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ox/2ox5_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ox5 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The bacterial Sox (sulfur oxidizing) system allows the utilization of inorganic sulfur compounds in energy metabolism. Central to this process is the SoxYZ complex that carries the pathway intermediates on a cysteine residue near the C terminus of SoxY. Crystal structures have been determined for Paracoccus pantotrophus SoxYZ with the carrier cysteine in the underivatized state, conjugated to the polysulfide mimic beta-mercaptoethanol, and as the sulfonate adduct pathway intermediate. The carrier cysteine is located on a peptide swinging arm and is bracketed on either side by diglycine dipeptides acting as molecular universal joints. This structure provides a novel solution to the requirement that the cysteine-bound intermediates be able to access and orient themselves within the active sites of multiple partner enzymes. Adjacent to the swinging arm there is a conserved, deep, apolar pocket into which the beta-mercaptoethanol adduct extends. This pocket would be well suited to a role in protecting labile pathway intermediates from adventitious reactions.


==About this Structure==
The SoxYZ complex carries sulfur cycle intermediates on a peptide swinging arm.,Sauve V, Bruno S, Berks BC, Hemmings AM J Biol Chem. 2007 Aug 10;282(32):23194-204. Epub 2007 May 23. PMID:17522046<ref>PMID:17522046</ref>
2OX5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Paracoccus_denitrificans Paracoccus denitrificans] with <scene name='pdbligand=ACT:'>ACT</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OX5 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
The SoxYZ Complex Carries Sulfur Cycle Intermediates on a Peptide Swinging Arm., Sauve V, Bruno S, Berks BC, Hemmings AM, J Biol Chem. 2007 Aug 10;282(32):23194-204. Epub 2007 May 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17522046 17522046]
</div>
<div class="pdbe-citations 2ox5" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Paracoccus denitrificans]]
[[Category: Paracoccus denitrificans]]
[[Category: Protein complex]]
[[Category: Berks BC]]
[[Category: Berks, B.C.]]
[[Category: Bruno S]]
[[Category: Bruno, S.]]
[[Category: Hemmings AM]]
[[Category: Hemmings, A.M.]]
[[Category: Sauve V]]
[[Category: Sauve, V.]]
[[Category: ACT]]
[[Category: EDO]]
[[Category: SO4]]
[[Category: immunoglobulin-like beta-sandwich fold]]
 
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Retrieved from "https://proteopedia.org/2ox5"