2y5y: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| (12 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
==Crystal structure of LacY in complex with an affinity inactivator== | |||
<StructureSection load='2y5y' size='340' side='right'caption='[[2y5y]], [[Resolution|resolution]] 3.38Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2y5y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y5Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y5Y FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.38Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=TGA:METHANETHIOSULFONYL-GALACTOSIDE'>TGA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y5y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y5y OCA], [https://pdbe.org/2y5y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y5y RCSB], [https://www.ebi.ac.uk/pdbsum/2y5y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y5y ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/LACY_ECOLI LACY_ECOLI] Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system). | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Lactose permease of Escherichia coli (LacY) with a single-Cys residue in place of A122 (helix IV) transports galactopyranosides and is specifically inactivated by methanethiosulfonyl-galactopyranosides (MTS-gal), which behave as unique suicide substrates. In order to study the mechanism of inactivation more precisely, we solved the structure of single-Cys122 LacY in complex with covalently bound MTS-gal. This structure exhibits an inward-facing conformation similar to that observed previously with a slight narrowing of the cytoplasmic cavity. MTS-gal is bound covalently, forming a disulfide bond with C122 and positioned between R144 and W151. E269, a residue essential for binding, coordinates the C-4 hydroxyl of the galactopyranoside moiety. The location of the sugar is in accord with many biochemical studies. | |||
Crystal structure of lactose permease in complex with an affinity inactivator yields unique insight into sugar recognition.,Chaptal V, Kwon S, Sawaya MR, Guan L, Kaback HR, Abramson J Proc Natl Acad Sci U S A. 2011 Jun 7;108(23):9361-6. Epub 2011 May 18. PMID:21593407<ref>PMID:21593407</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2y5y" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Lactose Permease|Lactose Permease]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Escherichia coli K-12]] | |||
[[Category: Large Structures]] | |||
[[Category: Abramson J]] | |||
[[Category: Chaptal V]] | |||
[[Category: Guan L]] | |||
[[Category: Kaback HR]] | |||
[[Category: Kwon S]] | |||
[[Category: Sawaya MR]] | |||
Latest revision as of 10:46, 20 December 2023
Crystal structure of LacY in complex with an affinity inactivator
| ||||||||||||
