2y8i: Difference between revisions
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==Structural basis for the allosteric interference of myosin function by mutants G680A and G680V of Dictyostelium myosin-2== | |||
<StructureSection load='2y8i' size='340' side='right'caption='[[2y8i]], [[Resolution|resolution]] 3.13Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2y8i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y8I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y8I FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.132Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y8i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y8i OCA], [https://pdbe.org/2y8i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y8i RCSB], [https://www.ebi.ac.uk/pdbsum/2y8i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y8i ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/MYS2_DICDI MYS2_DICDI] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The cold-sensitive single-residue mutation of glycine 680 in the reactive thiol region of Dictyostelium discoideum myosin-2 or the corresponding conserved glycine in other myosin isoforms has been reported to interfere with motor function. Here we present the x-ray structures of myosin motor domain mutants G680A in the absence and presence of nucleotide as well as the apo structure of mutant G680V. Our results show that the Gly-680 mutations lead to uncoupling of the reactive thiol region from the surrounding structural elements. Structural and functional data indicate that the mutations induce the preferential population of a state that resembles the ADP-bound state. Moreover, the Gly-680 mutants display greatly reduced dynamic properties, which appear to be related to the recovery of myosin motor function at elevated temperatures. | |||
Structural Basis for the Allosteric Interference of Myosin Function by Reactive Thiol Region Mutations G680A and G680V.,Preller M, Bauer S, Adamek N, Fujita-Becker S, Fedorov R, Geeves MA, Manstein DJ J Biol Chem. 2011 Oct 7;286(40):35051-60. Epub 2011 Aug 13. PMID:21841195<ref>PMID:21841195</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2y8i" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Myosin 3D Structures|Myosin 3D Structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Dictyostelium discoideum]] | |||
[[Category: Large Structures]] | |||
[[Category: Adamek N]] | |||
[[Category: Bauer S]] | |||
[[Category: Fedorov R]] | |||
[[Category: Fujita-Becker S]] | |||
[[Category: Geeves MA]] | |||
[[Category: Manstein DJ]] | |||
[[Category: Preller M]] | |||
Latest revision as of 08:10, 23 August 2023
Structural basis for the allosteric interference of myosin function by mutants G680A and G680V of Dictyostelium myosin-2
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